Guide to Protein Purification

Volume 182: Guide to Protein Purification
Erscheinungsjahr 1990
ISBN: 978-0-12-213585-9
Verlag: Elsevier Science & Technology

Buch, Englisch, 894 Seiten, Format (B × H): 178 mm x 241 mm

The Guide to Protein Purification is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher.

Key - Contains procedures newly written for this volume
- Provides extensive practical information
- Clearly describes rationale and strategies for protein and enzyme purification
- Offers personal perspectives on enzyme purification by eminent researchers

Autoren/Hrsg.

Abelson, John N.

Simon, Melvin I.

Deutscher, Murray P.

Fachgebiete

• Naturwissenschaften Agrarwissenschaften Agrarwissenschaften Landwirtschaftliche Bewässerung
• Wirtschaftswissenschaften Wirtschaftssektoren & Branchen Primärer Sektor Agrarökonomie, Ernährungswirtschaft
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Enzymologie
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung

Inhaltsverzeichnis

Developing Purification Procedures.
General Methods for Handling Proteins and Enzymes.
Optimization of Starting Materials.
Preparation of Extracts and Subcellular Fractionation.
Solubilization Procedures.
Purification Procedures: Bulk Methods.
Purification Procedures: Chromatographic Methods.
Purification Procedures: Electrophoretic Methods.
Specialized Purification Procedures.
Characterization of Purified Proteins.
Immunological Procedures.
Additional Techniques.
Concluding Remarks.
Personal Perspectives on Enzyme Purification.
Index.