Boheler / Gundry The Surfaceome

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-7553-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1722, 348 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Boheler, Kenneth R.

Gundry, Rebekah L.

Inhaltsverzeichnis

Preface…Table of Contents…Contributing Authors…

Part I Discovery-Based Approaches to Surfaceome Content
1. Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis AntigensYulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees, David Schieltz, and John R. Barr
2. “Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface ProteinsManuel J. Rodríguez-Ortega
3. Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial GenesWitold W. Kilarski, John Herbert, and Andreas Bikfalvi 
4. Mass Spectrometry Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and their DerivativesChelsea M. Fujinaka, Matthew Waas, and Rebekah L. Gundry
5. Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and its Proteome from Cells in SuspensionRebecca L. Rose, Waeowalee Choksawangkarn, and Catherine Fenselau
6. Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned MediaAnkit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, and Thomas Kislinger
Part II Targeted Approaches for Surfaceome Content
7. Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-phosphate Receptor, from Sf9 Cells Using the Baculovirus SystemLinda J. Olson and Nancy M. Dahms
8. Bispecific Antibody Armed T Cells to Target Cancer CellsArchana Thakur, Lawrence G. Lum, and Sandeep Mittal
9. Immunophenotyping of Live Human Pluripotent Stem Cells by Flow CytometryDaniel R. Riordon and Kenneth R. Boheler
10. Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4 Amanda M. Nevins and Adriano Marchese
Part III Cell-Based Functional Analyses Related to Surfaceome Content 
11. NaV Channels: Assaying Biosynthesis, Trafficking, FunctionGordon F. Tomaselli and Federica Farinelli
12. High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs)Chi-Wing Kong, Lin Geng, and Ronald A. Li
13. Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel DrugsYung Wui Tjong and Xiaoqiang Yao
14. Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in NeuronsKwok-On Lai and Nancy Y. Ip
15. Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma MembranesVladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, and Kapil Bharti
16. Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells under FlowTeresa Massam-Wu, Stuart A. Cain, and Catherine M. Kielty
17. Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses Jiaozi He, Zhihui Weng, Stanley Chun Ming Wu, and Kenneth R. Boheler
18. Fabrication and Mechanical Properties Measurements of 3D Microtissues for the Study of Cell-Matrix InteractionsPrasenjit Bose, Chen Yu Huang, Jeroen Eyckmans, Christopher S. Chen, and Daniel H. Reich
Part IV Computational Approaches in Surfaceome Studies
19. Discovery of Surface Target Proteins Linking Drugs, Molecular Markers, Gene Regulation, Protein Networks, and Disease by Using a Web-Based Platform Targets-SearchBin Yan, Pangwen Wang, Junwen Wang, and Kenneth R. Boheler