Buch, Englisch, Band 2550, 500 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 960 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2550, 500 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 960 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-2595-8
Verlag: Springer US
This volume covers the latest techniques that study the synthesis of melatonin, its receptor function, and its effects at the cellular and systemic level. The chapters are organized into three parts. Part One describes methods for the detection of melatonin and its biological derivatives in various biological samples, the manipulation of melatonin synthesis by the pineal gland in animals, and the principal source of melatonin in mammals. Part Two explores methods to measure the biological effects and consequences of melatonin binding to high-affinity G protein-coupled receptors. Part Three describes methods to measure the physiological effects that are regulated by melatonin in animals, particularly in rodent models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Melatonin: Methods and Protocols is a valuable resource for any researcher interested in investigating melatonin, from its production to its mechanisms of action and systemic effects.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Interdisziplinäres Wissenschaften Wissenschaften Interdisziplinär Neurowissenschaften, Kognitionswissenschaft
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft
- Naturwissenschaften Biowissenschaften Biowissenschaften
Weitere Infos & Material
Preface…
Table of Contents…
Contributing Authors…
Part I Methods for the Detection of Melatonin and Its Biological Derivatives
1. Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compounds
David C. Klein
2. Detection of Melatonin in Tissue Samples
Céline Legros and Jean A. Boutin
3. Measuring Dim Light Melatonin Onset in Humans
Melissa A. St. Hilaire and Steven W. Lockley
4. Measuring Urinary 6-sulphatoxymelatonin in Humans
Melissa A. St. Hilaire and Steven W. Lockley
5. MEL-Index: Estimation of Tissue Melatonin Levels using Gene Expression Data
Pedro Augusto CM Fernandes, Regina P. Markus, and Gabriela Sarti Kinker
6. Melatonin Synthesis Enzymes Activity: Radiometric Assays for AANAT, ASMT, and TPH
Fernanda Gaspar do Amaral, José Cipolla-Neto, and Solange Castro Afeche
7. Pinealectomy in Rats
Camila Congentino Gallo, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, and José Cipolla-Neto
8. Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats
Sarosh Irfan Madhani, David C. Klein, Estela M. Muñoz, and Luis E. Savastano
9. Pineal Microdialysis
Caroline Aparecida Pereira de Souza, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, and José Cipolla-Neto
10. Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland
Martin Fredensborg Rath and Morten Møller
11. Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-Culture
Solange Castro Afeche, Diego de Piazza Pimentel, Luís Felipe Ferro, and José Cipolla-Neto
12. Pineal Gland Culture
Solange Castro Afeche, Fernanda Gaspar do Amaral, and José Cipolla-Neto
13. Preparation of Single Pineal Cells
Steven L. Coon and David C. Klein
14. RNA Sequencing of Single Pineal CellsSteven L. Coon and David C. Klein
15. siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells
Aurea Susana Blancas-Velazquez and Martin Fredensborg Rath
16. Analysis of the Hu