Buch, Englisch, 368 Seiten, Format (B × H): 186 mm x 264 mm, Gewicht: 908 g
Buch, Englisch, 368 Seiten, Format (B × H): 186 mm x 264 mm, Gewicht: 908 g
ISBN: 978-1-394-28001-8
Verlag: John Wiley & Sons Inc
Provides a timely overview of the use of CRISPR and non-coding RNA technologies to develop climate-resilient crops
With mounting challenges from climate change, expanding populations, and resource limitations, the need for resilient and sustainable agricultural systems has never been greater. Genome and Epigenome Editing for Stress-Tolerant Crops summarizes advanced techniques for creating crops that can withstand both biotic and abiotic stressors. Edited by renowned biologist Jen-Tsung Chen, this authoritative volume discusses the coordination of CRISPR/Cas technology with ncRNA-based epigenetics to enhance stress tolerance and improve crop quality.
In addition to offering insights into genetic and molecular advances, contributions by experts in the field present key methodologies and applications that bridge multiple omics technologies with genome editing for impactful agricultural outcomes. Addressing emerging tools and strategies that could be instrumental in achieving the United Nations Sustainable Development Goals (SDGs) and advancing sustainable agriculture, Genome and Epigenome Editing for Stress-Tolerant Crops: - Provides an in-depth overview of CRISPR/Cas and non-coding RNA strategies to develop stress-tolerant crops.
- Integrates multiple omics approaches, including genomics, transcriptomics, and metabolomics for comprehensive crop improvement.
- Discusses strategies for resilience against both abiotic and biotic stressors, such as drought, salinity, pests, and pathogens.
- Offers practical applications of CRISPR and RNA technologies for high-yield, high-quality crop development.
- Presents recent research advancements in epigenetic regulation to fine-tune plant stress responses.
- Discusses future directions in plant science to inspire new research and experimental designs.
Genome and Epigenome Editing for Stress-Tolerant Crops is essential reading for advanced undergraduate and graduate courses in plant biology, molecular genetics, and agricultural biotechnology. It is also a valuable reference for researchers, plant breeders, and scientists working on crop improvement and climate-resilient agriculture initiatives.
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Weitere Infos & Material
List of Contributors xvii
Preface xxiii
1 Mitigating Heat Stress Response in CRISPR/Cas-Mediated Edited Crops by Altering the Expression Pattern of Noncoding DNA 1
Emanpreet Kaur, Louie Cris Lopos, and Andriy Bilichak
1.1 Introduction 1
1.2 Impact of Climate Change and Heat Stress on Crop Productivity 2
1.3 Strategies for Mitigating Heat Stress Response Through Gene Editing and Derivative Technologies 8
1.4 Conclusions and Future Perspectives 17
References 18
2 Design Future Crops with Stress Resilience by CRISPR/Cas Reprogramming Noncoding RNAs 33
Hidam Bishworjit Singh, Niraj Kumar, Riwandahun Marwein, Ajay Kumar Keot, Manash Pratim Sarmah, and Dhanawantari L. Singha
2.1 Introduction 33
2.2 Noncoding RNAs Associated with Drought in Plants 35
2.3 Noncoding RNA Associated with Salt Stress Tolerance in Plant 35
2.4 Important to Edit Noncoding RNA by CRISPR/Cas to Reprogram Them 36
2.5 Gene-Editing Mechanism in Plants with CRISPR/Cas Technology 37
2.6 Functions of Different Cas Proteins 39
2.7 Different Cas9 Variants for Genome Editing in Plants 40
2.8 Success in CRISPR/Cas-Editing Noncoding RNA Genes in Plants 41
2.9 Challenges Associated with Editing Noncoding RNA in Plants 41
2.10 Conclusion and Future Directions 43
References 43
3 Improving Plant Abiotic Stress Tolerance by Modulating LncRNAs Using CRISPR/Cas9 Technology 49
Muhammad Waseem, Sana Basharat, Iffat Shaheen, Liu Pingwu, Muhammad Shareef, and Nimra Shahid
3.1 Introduction 49
3.2 The CRISPR-Cas Technology 50
3.3 Mechanistic Overview of CRISPR/Cas9-Based Editing 52
3.4 Impact of lncRNAs on Plant Abiotic Stress 54
3.5 CRISPR/Cas lncRNA Editing Improves Plant Abiotic Stress Tolerance 57
3.6 Future Directions and Perspective 58
3.7 Conclusion 59
References 59
4 CRISPR/Cas-Modified Long Noncoding RNAs for Regulating Plant Abiotic Responses 65
Diliane Harumi Yaguinuma, Jardel de Oliveira, Fernanda Freitas de Oliveira, and Tiago Benedito dos Santos
4.1 Introduction 65
4.2 Long Noncoding RNA (lncRNA) from Plants May Also be Related to Stress 68
4.3 Conclusion 74
References 74
5 Functional Analysis of Plant Noncoding Genomes UsingCRISPR-Cas9-Mediated Approaches 87
Anna Rera, Privilege Chikove, Nyashadzashe Shengezi, Angeline Jurry, and Jyoti Prakash Sahoo
5.1 Introduction 87
5.2 Noncoding Genome in Plants 88
5.3 CRISPR-Cas9 Technology 90
5.4 Applications of CRISPR-Cas9 in Plant Genomics 91
5.5 Designing CRISPR-Cas9 Experiments for Noncoding Regions 93
5.6 Techniques for Characterizing CRISPR-Cas9-Mediated Mutations 94
5.7 Future Prospects 97
5.8 Conclusion 97
References 97
6 Small RNA-Mediated Plant Protection Constructed by CRISPR/Cas Genome Editing 101
Alagu Manickavelu
6.1 Introduction 101
6.2 Principles of Small RNA-Mediated Plant Defense Mechanisms 102
6.3 CRISPR/Cas-Mediated Engineering of Small RNA Pathways 104
6.4 Case Studies: Small RNA-Mediated Plant Protection Enhanced by CRISPR/Cas 106
6.5 Challenges and Limitations 108
6.6 Future Prospects 109
6.7 Conclusion 110
References 111
7 Plant Immunity Released by CRISPR/Cas Technology-Mediated Modifying Long Noncoding RNAs 115
Ananya Choudhury, Pritilagna Panigrahi, Swatismita Deo, Bhupati Nayak, Dhaarani V., Swarnalata Tripathy, Jyoti Prakash Sahoo, and Swapan Kumar Tripathy
7.1 Introduction 115
7.2 Plant Immunity 116
7.3 Long Noncoding RNAs and CRISPR/Cas Technology 116
7.4 Enhancing Plant Immunity Through lncRNA Modification 117
7.5 Key Roles of lncRNAs in Plant Immunity 118
7.6 Application of CRISPR/Cas Technology-Mediated Modifying Long Noncoding RNAs 118
7.7 Ethical Regulations, Bioethical Uses, and Future Prospects 119
7.8 Conclusion 121
References 122
8 Plant Stress Memory Rewrite by CRISPR/Cas-Mediated Approaches 127
Srutirekha Mishra, Subhashree Priyadarshinee Sahoo, Subhalaxmi Swain, Laxmipriya Pati, Dhaarani V., Swarnalata Tripathy, Jyoti Prakash Sahoo, and Swapan Kumar Tripathy
8.1 Introduction 127
8.2 Understanding Plant Stress Response 128
8.3 Plant Stress Memory and Its Affecting Factors 129
8.4 Molecular and Epigenetic Mechanism of Plant Stress Memory 130
8.5 Exploring CRISPR/Cas Approaches to Write Plant Stress Memory 131
8.6 Genome-Editing Technique Based on CRISPR/Cas 9 131
8.7 The CRISPR-Cas Genome Modification Mechanism 132
8.8 Ethical Issues 134
8.9 Future Prospects 135
8.10 Conclusion 135
References 135
9 Salinity-Tolerant Crop Breeding Through Reprogramming Noncoding RNAs Mediated by CRISPR/Cas9 Technology 141
Santanu Samanta and Aryadeep Roychoudhury
9.1 Introduction 141
9.2 Negative Impact of Salinity Stress on Plants 142
9.3 Why Developing Salinity-Tolerant Crops Is a Necessity? 143
9.4 Breeding for Salt-Tolerant Crops Against Salinity Stress 143
9.5 An Overview of ncRNAs in Plants 144
9.6 Mechanistic Insights of CRISPR/Cas9 Tool 147
9.7 Targeting/Reprogramming ncRNAs Using CRISPR/Cas9 Tools 148
9.8 Concluding Remarks 149
References 150
10 Genetic Engineering of Cis-Regulatory Elements by CRISPR/Cas Technology for Crop Improvement 157
Afreen Anis, Soumyashree Dash, Soumita Karmakar, and Jyoti Prakash Sahoo
10.1 Introduction 157
10.2 CRISPR/Cas System Advancements 157
10.3 CRISPR/Cas9 Application for Improving Crop Quality 159
10.4 Cis-regulatory Components in Introns, Promoters, and Intergenic Areas 160
10.5 Natural Variation Affecting Cis-Regulatory Elements in Crops by InDels, SNPs, and Transposable Elements 161
10.6 Ways to Use the CRISPR/Cas-Editing System for Transgene-Free Genetic Engineering 163
10.7 The Genome-Wide Association Study’s (GWAS) Identification of CREs that are Vital to Agriculture 163
10.8 Customized CRE Alterations 163
10.9 CRE Editing with CRISPR/Cas 165
10.10 Methods for Improving Horticultural Crops Through CRISPR/Cas-Mediated Cis-Engineering Applications167
10.11 Opportunities and Challenges 168
10.12 Conclusion 171
References 171
11 Harnessing CRISPR Technology to Edit Noncoding RNAs for Enhanced Disease Resistance in Crops 179
Abhik Sarkar, Subham Ghosh, Dipro Sinha, Parinita Das, Sneha Murmu, and Himanshushekhar Chaurasia
11.1 Introduction 179
11.2 A Brief Overview of CRISPR/Cas Technology 181
11.3 Overview of Noncoding RNAs 187
11.4 CRISPR/Cas-Edited Crops for Disease Resistance and Limitations 190
11.5 Modulation of Noncoding RNAs in CRISPR/Cas-Edited Crops 193
11.6 Example of CRISPR-Cas-Edited Crops with Modified Noncoding RNAs 196
11.7 Future Perspectives and Challenges 197
11.8 Conclusion 198
References 198
12 Temperature-Smart Crops Through Edited Noncoding RNAs Using CRISPR/Cas Technology 203
Sharia Yesmin Labonno, Marium Khatun, Sumi Sarkar, and Abul Kalam Mohammad Aminul Islam
12.1 Introduction 203
12.2 The Function of ncRNAs in Response to Temperature Stress in Plants 204
12.3 CRISPR/Cas Technology for Editing Noncoding RNAs 206
12.4 CRISPR/Cas9 Editing of Noncoding RNAs (ncRNAs) for Cold and Heat Tolerance 208
12.5 Applications in Cold and Heat Tolerance 209
12.6 Case Studies: Temperature-Smart Crops Developed Through ncRNA Editing 209
12.7 Challenges and Future Directions 212
12.8 Future Prospects for Temperature-Smart Crops 213
12.9 Conclusions 215
References 216
13 Technical Advancements in Functional Mutagenesis of Plant Long Noncoding RNAs Using CRISPR/Cas Technology 219
Tushar K. Dutta
13.1 Introduction 219
13.2 The Complex Architecture of Plant lncRNAs 220
13.3 The Diverse and Dynamic Functions of Plant lncRNAs 221
13.4 Utility and Challenges of CRISPR/Cas System for Deciphering lncRNA Functions 225
13.5 Functional Analysis of Different Plant lncRNAs Using CRISPR/Cas System 228
13.6 Conclusion and Future Directions 231
References 232
14 Plant Gene Silencing Modified by CRISPR/Cas-Reprogrammed Small Interfering RNAs for Regulating Abiotic Stress Responses 239
Noru Rajasekhar Reddy, Janapareddy Rajesh, Kadiyala Kavya, Lellapalli Rithesh, Abhishek Kumar, and Pooja
14.1 Introduction 239
14.2 CRISPR/Cas System: Principles and Applications 242
14.3 Role of Small Interfering RNAs in Gene Silencing 247
14.4 CRISPR/Cas-Mediated Reprogramming of siRNAs for Gene Silencing 249
14.5 Regulation of Abiotic Stress Responses Through Gene Silencing 250
14.6 Conclusion 254
References 255
15 Functional Analysis and Modification of Plant MicroRNA by CRISR/Cas System for Stress Tolerance 265
Rida Zahid, Ifrah Imran, Muhammad Waseem Sajjad, Muhammad Arslan Mahmood, Anam Ishtiaq, Saqib Siddique, Rabia Rehman, Imran Amin, and Rubab Zahra Naqvi
15.1 Introduction 265
15.2 miRNA Role in Plants 266
15.3 In Silico Approaches to Identifying miRNA 271
15.4 CRISPR-Based Editing of miRNA in Plants for Stress Control 278
15.5 Conclusion and Future Prospects 283
References 283
16 Employment of RNA Interference and CRISPR/Cas Machinery for Disease Resistance in Crops 293
Ifrah Imran, Muhammad Jawad Akbar Awan, Mariam Akhtar, Muhammad Waseem Sajjad, Muhammad Ismail Buzdar, Rida Zahid, Jahan Khan, Imran Amin, and Rubab Zahra Naqvi
16.1 Introduction 293
16.2 The Editors of Nature 295
16.3 The Mechanisms of Silence: Understanding RNA Interference 299
16.4 Advancing Agriculture: Engineered RNA Interference for Enhanced Crop Performance 301
16.5 RNAi Approaches: Targeted Silence to Combat Crop Diseases 302
16.6 Sculpting Genetic Frontiers: CRISPR and Its Triumphs in Crop Disease Management 306
16.7 Conclusion 310
References 312
17 Antiabiotic Stress Capacities of Crops Gained by CRISPR/Cas-Edited Small RNAs 321
Arfa Saifullah, Asad Azeem, Ghulam Mustafa, Muhammad Sarwar Khan, Sehrish Shehzadi, Aqsa Parvaiz, Maria Rehman, and Faiz Ahmad Joyia
17.1 Introduction 321
17.2 CRISPR/Cas Technology: Mechanism and Applications 322
17.3 CRISPR/Cas-Mediated Editing of sRNA for Stress Tolerance 324
17.4 Application of CRISPR/Cas-Edited sRNA in Stress Tolerance 325
17.5 Challenges and Future Prospects of CRISPR/Cas-Edited Small RNAs for Abiotic Stress Resistance in Crops 327
17.6 Conclusion 329
References 330
Index 335
