Clote | RNA Structure, Kinetics, and Rational Design | Buch | 978-1-032-49666-5 | www.sack.de

Buch, Englisch, 472 Seiten, Format (B × H): 210 mm x 280 mm

Reihe: Chapman & Hall/CRC Computational Biology Series

Clote

RNA Structure, Kinetics, and Rational Design

Volume Two
1. Auflage 2026
ISBN: 978-1-032-49666-5
Verlag: Taylor & Francis Ltd

Volume Two

Buch, Englisch, 472 Seiten, Format (B × H): 210 mm x 280 mm

Reihe: Chapman & Hall/CRC Computational Biology Series

ISBN: 978-1-032-49666-5
Verlag: Taylor & Francis Ltd


Volumes 1 and 2 of “RNA: Computational methods for structure, kinetics, and rational design” together constitute a comprehensive presentation of RNA secondary structures. Volume 1 concerns thermodynamics, energy parameters, methods to count structures, base pairs, loops, leading to many algorithms concerning RNA structure.Volume 2 covers secondary structure folding kinetics, starting with an inquiry into whether the ensemble of RNA structures is small-world or scale-free, then proceeding to the identification of local energy minima that create a basin of attraction, leading to a coarse-grained kinetics model. A novelty is the presentation of Markov state models (MSM) and Transition Path Theory (TPT), developed originally to create coarse-grained kinetics models for (especially) proteins from many independent molecular dynamics trajectories. The book describes Markov state models created from secondary structure folding trajectories generated by the kinetic Monte Carlo (Gillespie) algorithm. As this is the first such application in the literature, our Python code will be made available at the book’s website, with the intent that other research teams might use the code to extend this approach. Current RNA research concerns the rational design of functional RNA molecules, which depends on inverse folding algorithms that generate many sequences that (approximately) fold into a given target structure. For that reason, the book provides an overview and comparison of various inverse folding algorithms, which often depend on some form of combinatorial optimization. The question of whether inverse folding is computationally hard (NP-complete) is addressed, and another chapter focuses on various types of designed RNA: ribozymes, RNA thermometers, bistable switches, toehold switches, mRNA vaccines, paper-based COVID-19 tests, etc. The final chapter is a math appendix for the linear algebra and Markov chain results that are essential to folding kinetics, especially in the case of Markov state models. In summary, volume 2 presents the necessary concepts from mathematics and algorithm theory for the next generation of research teams to extend the computational work on RNA, especially for the rational design of functional molecules – a topic that not so long ago seemed more like science fiction. Exercises at the end of each chapter often introduce new material or related concepts, and solutions are available at the book’s website.The book includes the first description in the literature (our code dates from Feb, Mar 2021) of the construction of Markov state models (MSM) and transition path theory (TPT) for secondary structure folding trajectories generated by the kinetic Monte Carlo algorithm. Other features include network properties (small-world, scale-free) of the ensemble of secondary structures, a succinct overview and comparison of inverse folding methods, discussion of whether inverse folding is NP-complete, design of ribozymes, thermoswitches, toehold switches, thermodynamically stable mRNA vaccines.

Clote RNA Structure, Kinetics, and Rational Design jetzt bestellen!

Zielgruppe


Postgraduate, Professional Reference, and Undergraduate Advanced


Autoren/Hrsg.


Weitere Infos & Material


1. RNA world hypothesis (Gilbert) and diverse roles of RNA in cell biology 2. Review of thermodynamics for ab initio secondary structure modeling 3. Energy parameters via optical melting 4. Dynamic programming thermodynamics-based algorithms for structure prediction a) Minimum free energy structure, and variants thereof b) Partition function, sampling low energy structures for biological applications c) Saturated and locally optimal structures, expected 5'-3' distance 5. Coarse-grained kinetics via basins of attraction a) Gillespie's algorithm b) Kinfold, KFOLD, and related algorithms 6. Markov state models for secondary structure kinetics 7. Rational design of RNA structures a) Inverse folding problem b) Ribozyme and riboswitch design Appendix: Review of linear algebra and Markov processes for applications in kinetics


A Fulbright and Guggenheim fellow, Dr. Clote has held faculty positions in Mathematics, Computer Science and Biology in France, Germany and the United States, including the Gentzen Chair of Computer Science at the University of Munich, and the Digiteo Chair of Excellence at Ecole Polytechnique. This provides a unique background for the current interdisciplinary text on RNA.



Ihre Fragen, Wünsche oder Anmerkungen
Vorname*
Nachname*
Ihre E-Mail-Adresse*
Kundennr.
Ihre Nachricht*
Lediglich mit * gekennzeichnete Felder sind Pflichtfelder.
Wenn Sie die im Kontaktformular eingegebenen Daten durch Klick auf den nachfolgenden Button übersenden, erklären Sie sich damit einverstanden, dass wir Ihr Angaben für die Beantwortung Ihrer Anfrage verwenden. Selbstverständlich werden Ihre Daten vertraulich behandelt und nicht an Dritte weitergegeben. Sie können der Verwendung Ihrer Daten jederzeit widersprechen. Das Datenhandling bei Sack Fachmedien erklären wir Ihnen in unserer Datenschutzerklärung.