Natural Products From Marine Algae

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2015
ISBN: 978-1-4939-5273-1
Verlag: Springer

Buch, Englisch, Band 1308, 439 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 9526 g

Reihe: Methods in Molecular Biology

This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Connan, Solène

Stengel, Dagmar B.

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie
• Naturwissenschaften Biowissenschaften Meeresbiologie
• Naturwissenschaften Biowissenschaften Botanik Phykologie, Algen, Flechten, Lichen
• Naturwissenschaften Chemie Analytische Chemie Magnetresonanz

Inhaltsverzeichnis

1. Marine Algae a Source Of Biomass for Biotechnological Applications

Dagmar B Stengel and Solène Connan

2. Structure and Function of Macro algal Natural Products

Ryan M. Young, Kathryn M. Schoenrock, Jacqueline L. von Salm, Charles D. Amsler, and Bill J. Baker

3. Spectrophotometric Assays of Major Compounds Extracted From Algae

Solène Connan

4. Extraction and Enrichment of Protein From Red and Green Macroalgae

Pádraigín A Harnedy and Richard. J. FitzGerald

5. Extraction and Purification of R-phycoerythrin from Marine Red Algae

Justine Dumay, Michèle Morançais, Trang Nguyen Huu Phuo, and Joël Fleurence

6. Extraction and Analysis of Mycosporine-like Amino Acids in Marine Algae 

Nedeljka N. Rosic, Christoph Braun, and David Kvaskoff

7. Extraction and Purification Of Phlorotannins from Brown Algae

Erwan Ar Gall, Florian Lelchat, Mélanie Hupel, Camille Jégou, and Valérie Stiger-Pouvreau

8. Enzyme-enhanced Extraction Of Antioxidant Ingredients From Algae

Björn V. Adalbjörnsson and Rósa Jónsdóttir

9. Microwave-assisted Extraction Of Fucoidan From Marine Algae

Solange I. Mussatto

10. Extraction and Analysis of Oxylipins from Macroalgae Illustrated on the Example Gracilaria vermiculophylla

Dominique Jacquemoud and Georg Pohnert

11. Lipids and Fatty Acids In Algae: Extraction, Fractionation Into Lipid Classes, and Analysis by Gas Chromatography Coupled with Flame Ionisation Detector (GC-FID)

Freddy Guihéneuf, Matthias Schmid, and Dagmar B. Stengel

12. HRMAS NMR Analysis Of Algae and Identification of Molecules of Interest Via Conventional 1D and 2D NMR: Sample Preparation and Optimization Of Experimental Conditions

Gaëlle Simon, Nelly Kervarec, and Stéphane

Cérantola

13. Extraction, Purification and NMR Analysis Of Terpenes From Brown Algae

Marc Gaysinski, Annick Ortalo-Magné, Olivier P. Thomas, and Gérald Culioli

14. Extraction, Isolation, and Identification of Sesquiterpenes From Laurencia Species

Angélica Ribeiro Soares

15. The use of HPLC for the Characterisation Of Phytoplankton Pigments

José L. Garrido and Suzanne Roy

16. Characterization of Phlorotannins from Brown Algae by LC-HRMS

Jeremy E. Melanson and Shawna L. MacKinnon

17. Analysis of Betaines From Marine Algae using LC-MS-MS

Shawna L. MacKinnonand Cheryl Craft

18. Analysis of Marine Biotoxins using LC-MS/MS

Bernd Luckas, Katrin Erler, and Bernd Krock

19. Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry

Stanislav D. Anastyuk, Natalia M. Shevchenko, and Vladimir I. Gorbach

20. Determination of Substitution Patterns of Galactans from Green Seaweeds Of The Bryopsidales

Paula Ximena Arata, Paula Virginia Fernández, and Marina Ciancia

21. Structural Characterization Of A Hybrid Carrageenan-Like Sulphated Galactan from a Marine Red Alga Furcellaria lumbricalis

Youjing Lv, Bo Yang, Xia Zhao, Junzeng Zhang, and Guangli Yu

22. Characterisation Of Alginates By Nuclear Magnetic Resonance (NMR) and Vibrational Spectroscopy (IR, NIR, Raman) in Combination with Chemometrics

Henrik Max Jensen, Flemming Hofmann Larsen, and Søren Balling Engelsen

 23. Imaging and Identification of Marine Algal Bio-active Compounds by Surface Enhanced Raman Spectroscopy (SERS)

Mats Josefson, Alexandra Walsh, and Katarina Abrahamsson

 24. In vitro protocols for Measuring the Antioxidant Capacity of Algal Extracts

Owen Kenny, Nigel P. Brunton, and Thomas J. Smyth

25. Disk

Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts

Andrew P. Desbois and Valerie J. Smith

26. Screening of a Marine Algal Extract for Antifungal Activities

Graciliana Lopes, Paula B. Andrade, and Patrícia Valentão

27. Protocol for Assessing Antifouling Activities of Macroalgal Extracts

Claire Hellio, Rozenn Trepos , R. Noemí Aguila-Ramírez, and Claudia J. Hernández-Guerrero