1. Auflage 2022,
Band: 2402, 300 Seiten, Gebunden, Book, Format (B × H): 183 mm x 260 mm, Gewicht: 787 g
Reihe: Methods in Molecular Biology
Verlag: HUMANA PR
Cranfield Membrane Lipids: Methods and ProtocolsThis detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.
Weitere Infos & Material
1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang 2. Preparing Ion Channel Switch Membrane-Based Biosensors Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela 3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt 4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes Tadas Penkauskas, Filipas Ambrulevicius, and Gintaras Valincius 5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster 6. Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs) Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster 7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield 8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer Kan Shoji 9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes 10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations Robby Manrique 11. Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins Amanda Buyan and Ben Corry 12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling Kathleen Wood 13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt 14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt 15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson 16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs) Kinga Burdach, Damian Dziubak, and Slawomir Sek 17. Manipulation of Lipid Membranes with Thermal Stimuli Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen 18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid beta Oligomers by Atomic Force Microscopy Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta 19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté 20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR Elvis Pandzic, Renee Whan, and Alex Macmillan 21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides Srikanth Dumpati and Debarun Dutta 22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi Maria Makarova and Dylan M. Owen