Plant Stem Cells

Methods and Protocols
1. Auflage 2020
ISBN: 978-1-0716-0182-2
Verlag: Springer US

Buch, Englisch, Band 2094, 150 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 518 g

Reihe: Methods in Molecular Biology

This volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Dandekar, Thomas

Naseem, Muhammad

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Botanik

Inhaltsverzeichnis

Preface…Table of Contents…Contributing Author… 
1. Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell NichesAqib Syed, Anwar Hussain, Waheed Murad, and Badshah Islam   
2. Bacterial Shoot Apical Meristem Inoculation Assay Muhammad Naseem, Gökhan Gun, Ozge Osmanoglu, Fatima A. AlRemeithi, Jibran Iqbal, Ihsan Ullah, and Thomas Dandekar
3. Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana using TAL-Effectors NucleasesJoachim Forner
4. Method to Study Gene Expression Patterns during de novo Root Regeneration from Arabidopsis Leaf ExplantsJie Yu, Ning Zhai, Lin Xu, and Wu Liu
5. Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture their Transcriptome Profile Monika Mahajan and Ram Kishor Yadav
6. Plant Associated Microbes Alter Root Growth by Modulating Root Apical MeristemAnwar Hussain, Husna Ullah, Ihsan Ullah, and Muhammad Naseem 
7. Live Imaging of Arabidopsis Axillary MeristemsBihai Shi, Hongli Wang, and Yuling Jiao
8. Molecular Modeling of the Interaction between Stem Cells Peptide and Immune Receptor in ArabidopsisMuhammad Naseem, Mugdha Srivastava, Ozge Osmanoglu, Jibran Iqbal, Fares M. Howari, Fatima A. AlRemeithi, and Thomas Dandekar
9. Methods to Visualize Auxin and Cytokinins Signaling Activity in the Shoot Apical MeristemGéraldine Brunoud, Carlos S. Galvan-Ampudia, and Teva Vernoux
10. Analysis of Cell Division Frequency in the Root Apical Meristem of Lycophytes, Non-Seed Vascular Plants, using EdU LabelingRieko Fujinami
11. Osmotic Treatment for Quantifying Cell Wall Elasticity in the Sepal of Arabidopsis thalianaAleksandra Sapala and Richard S. Smith
12. Mapping a Transcriptome Guided Arabidopsis SAM InteractomeMuhammad Naseem, Ozge Osmanoglu, Jibran Iqbal, Fares M. Howari, Fatima A. AlRemeithi, Martin Kaltdorf, and Thomas Dandekar
13. 3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS ToolboxViktoriya V. Lavrekha, Taras Pasternak, Klaus Palme, and Victoria V. Mironova
14. Micropropagation of Rosaceous Species SAM Grown in Temperate ClimateJiri Sedlak and Frantisek Paprstein

15. A New Perspective on Cryotherapy: Pathogen Elimination using Plant Shoot Apical Meristem via Cryogenic TechniquesErgun Kaya, Selin Galtali, Sevinc Gultag, and Onur Celik