Apoptosis Methods in Pharmacology and Toxicology

Approaches to Measurement and Quantification
1. Auflage. Softcover version of original hardcover Auflage 2002
ISBN: 978-1-61737-239-1
Verlag: Humana Press

Buch, Englisch, 156 Seiten, Paperback, Format (B × H): 152 mm x 229 mm, Gewicht: 259 g

Reihe: Methods in Pharmacology and Toxicology

Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.

Zielgruppe

Research

Autoren/Hrsg.

Davis, Myrtle A.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie

Inhaltsverzeichnis

Introduction
Myrtle A. Davis

Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death
Martin Poot, Robert H. Pierce, and Terrance J. Kavanagh

Analysis of Apoptosis by Laser Scanning Cytometry
Zbigniew Darzynkiewicz, Elzbieta Bedner, and Piotr Smolewski

Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections
Matthew A. Wallig, Curtis M. Chan, and Nancy A. Gillett

DNA Microarrays: An Overview of Technologies and Applications
Helmut Zarbl

Microarray Analysis of Apoptosis
Richard W. E. Clarkson, Catherine A. Boucher, and Christine J. Watson

ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases
Calvin F. Roff, Amy M. Walz, Lisa B. Niehoff, David J. Sdano, Antoinette M. Bennaars, Jeffrey A. Cooper, Becky L. Senft, Anatoli A. Sorkin, Steven P. Stoesz, and Paul A. Saunders

Index