Di Benedetto Astrocytes

Methods and Protocols
1. Auflage 2019
ISBN: 978-1-4939-9067-2
Verlag: Springer, Berlin

This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Di Benedetto, Barbara

Inhaltsverzeichnis

Part I: Astrocyte Biology: Overviews and Perspectives 1. The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse            Uzma Hasan and Sandeep K. Singh 2. Role of Astrocytes in the Neurogenic Niches            Julia Schneider, Julian Karpf, and Ruth Beckervordersandforth Part II: Isolation of Astrocytes from Animal Models 3. Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains            Yingbo He, Natalie Taylor, and Anindya Bhattacharya 4. Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon            Rossella Di Giaimo, Sven Aschenbroich, and Jovica Ninkovic Part III: Astrocyte Morphology and Functions: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro 5. A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ            Janosch P. Heller and Dmitri A. Rusakov 6. A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples            Gaël Quesseveur, Aymeric Fouquier d'Hérouël, Keith K. Murai, and David S. Bouvier 7. Cisterna Magna Injection in Rats to Study Glymphatic Function            Marta Ramos, Nicholas Burdon Bechet, Roberta Battistella, Chiara Pavan, Anna L.R. Xavier, Maiken Nedergaard, and Iben Lundgaard 8. Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface            Anne-Cécile Boulay, Noémie Mazaré, Bruno Saubaméa, and Martine Cohen-Salmon 9. Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission            Sara Mederos and Gertrudis Perea 10. Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping SystemOlga Barca Mayo, Luca Berdondini, and Davide De Pietri Tonelli 11. In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes            Youkyeong Gloria Byun and Won-Suk Chung 12. Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCd            Kiavasch M.N. Farid and Amin Derouiche 13. Imaging of Local and Global Sodium Signals in Astrocytes            Niklas J. Gerkau, Karl W. Kafitz, and Christine R. Rose 14. Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator            Ewa E. Bres and Andreas Faissner 15. Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes            Victoria Adelheid Malik, Barbara Di Benedetto, and Mira Jakovcevski Part IV: Astrocyte Pathologies in the Central Nervous System: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro 16. Imaging and Manipulating Astrocyte Function In Vivo in the Context of CNS Injury            Oleksii Shandra and Stefanie Robel 17. Glial Pathology in Major Depressive Disorder: An Approach to Investigate the Coverage of Blood Vessels by Astrocyte Endfeet in Human Postmortem Brain            Grazyna Rajkowska and Jose Javier Miguel-Hidalgo 18. Examining the Coverage of Blood Vessels by Astrocyte Endfeet in an Animal Model of Major Depressive Disorder            Heike Hallof-Büstrich and Barbara Di Benedetto 19. A Method to Investigate Astrocyte and Microglial Morphological Changes in the Aging Brain of the Rhesus Macaque            Kevin B. Chiu, Kim M. Lee, Katelyn N. Robillard, and Andrew G. MacLean 20. Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes            Gabor Heimann and Swetlana Sirko