Grant / Li Analysis of Post-Translational Modifications and Proteolysis in Neuroscience

1. Auflage 2016
ISBN: 978-1-4939-3472-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 114, 290 Seiten, eBook

Reihe: Neuromethods

This volume highlights proteomics studies of quantitative PTM changes in both peripheral and central nervous system proteomes utilizing the most recent advances in mass spectrometry. Chapters include practical information pertaining to the fundamentals of sample preparation, liquid chromatography, and tandem mass spectrometry instrumental analysis and will elucidate best practices in the interpretation of data using modern bioinformatics approaches. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience aimsto ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Grant, Jennifer Elizabeth

Li, Hong

Inhaltsverzeichnis

1. Proteomic Analysis of Post-Translational Modifications in Neurobiology Hongbo Gu, Matthew P. Stokes, and Jeffrey C. Silva 2. A Boronic Acid-based Enrichment for Site-specific Identification of the N-glycoproteome using MS-based Proteomics Haopeng Xiao, George X. Tang, Weixuan Chen, and Ronghu Wu 3. Site-specific Localization of D-Amino Acids in Bioactive Peptides by Ion Mobility Spectrometry Chenxi Jia, Christopher B. Lietz, Qing Yu, and Lingjun Li 4. Quantitative Profiling of Reversible Cysteome Modification under Nitrosative Stress Yue-Ting Wang, Sujeewa C. Piyankarage, and Gregory R. J. Thatcher 5. Label-free LC-MS/MS Comparative Analysis of Protein S-nitrosome in Synaptosomes from Wild Type and APP Transgenic Mice Monika Zareba-Koziol, Maciej Lalowski, and Aleksandra Wyslouch-Cieszynska 6. Proteomics Identification of Redox-Sensitive Nuclear Protein Targets Of Human Thioredoxin 1 from  SHSY-5Y Neuroblastoma Cell Line Mohit Raja Jain, Changgong Wu, Qing Li, Chuanlong Cui, Huacheng Dai, and Hong Li 7. Identification and Quantification of K63-Ubiquitinated Proteins in Neuronal Cells by High- Resolution Mass Spectrometry Gustavo Monteiro Silva, Wei Wei, Sandhya Manohar,  and Christine Vogel 8. Phosphorylation Site Profiling of NG108 Cells Using Quadrupole/Orbitrap Mass Spectrometry Fang-Ke Huang, Guoan Zhang, and Thomas A. Neubert  9. Phosphoproteomics of Tyrosine Kinases in the Nervous System Robert J. Chalkley and Ralph A. Bradshaw 10. Analysis of PINK1 and CaMKII Substrates Using Mass Spectrometry-Based Proteomics Yan Li, Lesley A. Kane, Michael A Bemben, and Katherine W Roche 11. Liquid Chromatography-Tandem Mass Spectrometry Analysis of Tau Phosphorylation Jhoana Mendoza,Georgia Dolios, and Rong Wang 12. Identification of Protease Substrates in Complex Proteomes by iTRAQ-TAILS Tobias Kockmann, Nathalie Carte, Samu Melkko, and Ulrich auf dem Keller 13. Proteolytic Processing of Neuropeptides Lloyd D. Fricker 14 Isotope Dilution Analysis of Myelin Basic Protein Degradation after Brain Injury Andrew K Ottens 15. Identification and Characterization of Protein Post-Translational Modifications by Differential Fluorescent Labeling Eric D. Hamlett, Cristina Osorio, and Oscar Alzate 16 Mass Spectrometric Detection of Detyrosination and Polyglutamyation on the C-Terminal Region of Brain Tubulin Yasuko Mori, Alu Konno, Mitsutoshi Setou, and Koji Ikegami 17. Determination of polyhydroxybutyrate (PHB) Post-Translational Modifications of Proteins Using Mass Spectrometry Tong Liu, Wei Chen, Stacey Pan, Chuanlong Cui, Hong Li, and Eleonora Zakharian