Herdewijn Oligonucleotide Synthesis

Methods and Applications
1. Auflage. Softcover version of original hardcover Auflage 2005
ISBN: 978-1-61737-441-8
Verlag: Springer, Berlin

Seite exportieren

Beschreibung

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Autoren

Herausgeber

Herdewijn, Piet

Über den Autor

Herdewijn, Piet
Piet Herdewijn is Professor at the Katholieke Universiteit Leuven, Belgium, and head of the laboratory of medicinal chemistry at the Rega Institute. He obtained his Ph.D. and habilitation from the same university. He was a postdoctoral fellow of the Humboldt Foundation at the University of Konstanz. He has conducted research in bioorganic and medicinal chemistry, mainly in the field of nucleosides, nucleotides, and nucleic acids, at the University of Leuven, the University of Ghent, and the Université d'Evry-Val-d'Essonne.

Inhaltsverzeichnis

Synthesis of DNA Using a New Two-Step Cycle
Douglas J. Dellinger, Jason R. Betley, Tadeusz K. Wyrzykiewicz, and Marvin H. Caruthers

RNA Synthesis Using 2'-O-(Tert-Butyldimethylsilyl) Protection
Brian S. Sproat

RNA Oligonucleotide Synthesis Via 5'-Silyl-2'-Orthoester Chemistry
Stephanie A. Hartsel, David E. Kitchen, Stephen A. Scaringe, and William S. Marshall

Dimethylthiarum Disulfide: New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide Synthesis
Zhiwei Wang, Quanlai Song, and Yogesh S. Sanghvi

Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes
Annie Galarneau, Kyung-Lyum Min, Maria M. Mangos, and Masad J. Damha

Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry
Jacek Stawinski and Roger Strömberg

Solid-Phase Synthesis of Circular Oligonucleotides
Enrique Pedroso, Nuria Escaja, Miriam Frieden, and Anna Grandas

Locked Nucleic Acid Synthesis
Henrik M. Pfundheller, Anders M. Sørensen, Christian Lomholt, Anders M. Johansen, Troels Koch, and Jesper Wengel

Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-oligomers
Vladimir A. Efimov and Oksana G. Chakhmakhcheva

Base-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-d]pyrimidines Replacing Purines
Frank Seela, Yang He, Junlin He, Georg Becher, Rita Kröschel, Matthias Zulauf, and Peter Leonard

Introduction of Hypermodified Nucleotides in RNA
Darrell R. Davis and Ashok C. Bajji

Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis
Dmitry A. Stetsenko and Michael J. Gait

Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms
A. Michael Morocho, Valeri Karamyshev, Olga Shcherbinina, and Nikolai Polushin

Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips
Ali Laayoun,Eloy Bernal-Méndez, Isabelle Sothier, Mitsuharu Kotera, and Alain Troesch

Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides
Alexandre S. Boutorine, and Jian-Sheng Sun

Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis
Ute Scheffer and Michael Göbel

Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays
Jacqueline A. M. Vet and Salvatore A. E. Marras

High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing
Nikolai Polushin, Andrei Malykh, A. Michael Morocho, Alexei Slesarev, and Sergei Kozyavkin

Nonenzymatic Template-Directed RNA Synthesis
Marcus Hey and Michael Göbel

DNAse I Footprinting of Small Molecule Binding Sites on DNA
Christian Bailly, Jérôme Kluza, Christopher Martin, Thomas Ellis, and Michael J. Waring

Gene Targeting Using Peptide Nucleic Acid
Peter E. Nielsen

Nucleic Acid Library Construction Using Synthetic DNA Constructs
Hani S. Zaher and Peter J. Unrau

In Vitro Selection From Combinatorial Nucleic Acid Libraries
Andres Jäschke

In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins
Eric Dausse, Christian Cazenave, Bernard Rayner, and Jean-Jacques Toulmé

Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism
Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy

Index

Zielgruppe

Research

Fachgebiete