Herdewijn Oligonucleotide Synthesis
1. Auflage. Softcover version of original hardcover Auflage 2005
ISBN: 978-1-61737-441-8
Verlag: Springer, Berlin
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Buch, Englisch, Reihe: Methods in Molecular Biology
Band: 288
436 Seiten, Kartoniert, Previously published in hardcover, Format (B × H): 154 mm x 228 mm, Gewicht: 687 g
Methods and Applications
1. Auflage. Softcover version of original hardcover Auflage 2005,
Band: 288, 436 Seiten, Kartoniert, Previously published in hardcover, Format (B × H): 154 mm x 228 mm, Gewicht: 687 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61737-441-8
Verlag: Springer, Berlin
Seite exportieren
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Herdewijn, Piet
Piet Herdewijn is Professor at the Katholieke Universiteit Leuven, Belgium, and head of the laboratory of medicinal chemistry at the Rega Institute. He obtained his Ph.D. and habilitation from the same university. He was a postdoctoral fellow of the Humboldt Foundation at the University of Konstanz. He has conducted research in bioorganic and medicinal chemistry, mainly in the field of nucleosides, nucleotides, and nucleic acids, at the University of Leuven, the University of Ghent, and the Université d'Evry-Val-d'Essonne.
Synthesis of DNA Using a New Two-Step Cycle
Douglas J. Dellinger, Jason R. Betley, Tadeusz K. Wyrzykiewicz, and Marvin H. Caruthers
RNA Synthesis Using 2'-O-(Tert-Butyldimethylsilyl) Protection
Brian S. Sproat
RNA Oligonucleotide Synthesis Via 5'-Silyl-2'-Orthoester Chemistry
Stephanie A. Hartsel, David E. Kitchen, Stephen A. Scaringe, and William S. Marshall
Dimethylthiarum Disulfide: New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide Synthesis
Zhiwei Wang, Quanlai Song, and Yogesh S. Sanghvi
Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes
Annie Galarneau, Kyung-Lyum Min, Maria M. Mangos, and Masad J. Damha
Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry
Jacek Stawinski and Roger Strömberg
Solid-Phase Synthesis of Circular Oligonucleotides
Enrique Pedroso, Nuria Escaja, Miriam Frieden, and Anna Grandas
Locked Nucleic Acid Synthesis
Henrik M. Pfundheller, Anders M. Sørensen, Christian Lomholt, Anders M. Johansen, Troels Koch, and Jesper Wengel
Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-oligomers
Vladimir A. Efimov and Oksana G. Chakhmakhcheva
Base-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-d]pyrimidines Replacing Purines
Frank Seela, Yang He, Junlin He, Georg Becher, Rita Kröschel, Matthias Zulauf, and Peter Leonard
Introduction of Hypermodified Nucleotides in RNA
Darrell R. Davis and Ashok C. Bajji
Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis
Dmitry A. Stetsenko and Michael J. Gait
Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms
A. Michael Morocho, Valeri Karamyshev, Olga Shcherbinina, and Nikolai Polushin
Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips
Ali Laayoun,Eloy Bernal-Méndez, Isabelle Sothier, Mitsuharu Kotera, and Alain Troesch
Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides
Alexandre S. Boutorine, and Jian-Sheng Sun
Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis
Ute Scheffer and Michael Göbel
Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays
Jacqueline A. M. Vet and Salvatore A. E. Marras
High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing
Nikolai Polushin, Andrei Malykh, A. Michael Morocho, Alexei Slesarev, and Sergei Kozyavkin
Nonenzymatic Template-Directed RNA Synthesis
Marcus Hey and Michael Göbel
DNAse I Footprinting of Small Molecule Binding Sites on DNA
Christian Bailly, Jérôme Kluza, Christopher Martin, Thomas Ellis, and Michael J. Waring
Gene Targeting Using Peptide Nucleic Acid
Peter E. Nielsen
Nucleic Acid Library Construction Using Synthetic DNA Constructs
Hani S. Zaher and Peter J. Unrau
In Vitro Selection From Combinatorial Nucleic Acid Libraries
Andres Jäschke
In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins
Eric Dausse, Christian Cazenave, Bernard Rayner, and Jean-Jacques Toulmé
Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism
Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy
Index
Research
Herdewijn, Piet
Piet Herdewijn is Professor at the Katholieke Universiteit Leuven, Belgium, and head of the laboratory of medicinal chemistry at the Rega Institute. He obtained his Ph.D. and habilitation from the same university. He was a postdoctoral fellow of the Humboldt Foundation at the University of Konstanz. He has conducted research in bioorganic and medicinal chemistry, mainly in the field of nucleosides, nucleotides, and nucleic acids, at the University of Leuven, the University of Ghent, and the Université d'Evry-Val-d'Essonne.
Synthesis of DNA Using a New Two-Step Cycle
Douglas J. Dellinger, Jason R. Betley, Tadeusz K. Wyrzykiewicz, and Marvin H. Caruthers
RNA Synthesis Using 2'-O-(Tert-Butyldimethylsilyl) Protection
Brian S. Sproat
RNA Oligonucleotide Synthesis Via 5'-Silyl-2'-Orthoester Chemistry
Stephanie A. Hartsel, David E. Kitchen, Stephen A. Scaringe, and William S. Marshall
Dimethylthiarum Disulfide: New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide Synthesis
Zhiwei Wang, Quanlai Song, and Yogesh S. Sanghvi
Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes
Annie Galarneau, Kyung-Lyum Min, Maria M. Mangos, and Masad J. Damha
Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry
Jacek Stawinski and Roger Strömberg
Solid-Phase Synthesis of Circular Oligonucleotides
Enrique Pedroso, Nuria Escaja, Miriam Frieden, and Anna Grandas
Locked Nucleic Acid Synthesis
Henrik M. Pfundheller, Anders M. Sørensen, Christian Lomholt, Anders M. Johansen, Troels Koch, and Jesper Wengel
Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-oligomers
Vladimir A. Efimov and Oksana G. Chakhmakhcheva
Base-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-d]pyrimidines Replacing Purines
Frank Seela, Yang He, Junlin He, Georg Becher, Rita Kröschel, Matthias Zulauf, and Peter Leonard
Introduction of Hypermodified Nucleotides in RNA
Darrell R. Davis and Ashok C. Bajji
Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis
Dmitry A. Stetsenko and Michael J. Gait
Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms
A. Michael Morocho, Valeri Karamyshev, Olga Shcherbinina, and Nikolai Polushin
Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips
Ali Laayoun,Eloy Bernal-Méndez, Isabelle Sothier, Mitsuharu Kotera, and Alain Troesch
Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides
Alexandre S. Boutorine, and Jian-Sheng Sun
Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis
Ute Scheffer and Michael Göbel
Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays
Jacqueline A. M. Vet and Salvatore A. E. Marras
High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing
Nikolai Polushin, Andrei Malykh, A. Michael Morocho, Alexei Slesarev, and Sergei Kozyavkin
Nonenzymatic Template-Directed RNA Synthesis
Marcus Hey and Michael Göbel
DNAse I Footprinting of Small Molecule Binding Sites on DNA
Christian Bailly, Jérôme Kluza, Christopher Martin, Thomas Ellis, and Michael J. Waring
Gene Targeting Using Peptide Nucleic Acid
Peter E. Nielsen
Nucleic Acid Library Construction Using Synthetic DNA Constructs
Hani S. Zaher and Peter J. Unrau
In Vitro Selection From Combinatorial Nucleic Acid Libraries
Andres Jäschke
In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins
Eric Dausse, Christian Cazenave, Bernard Rayner, and Jean-Jacques Toulmé
Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism
Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy
Index
Research
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