Techniques for Biochemical Analysis

Erscheinungsjahr 2024
ISBN: 978-0-443-15914-5
Verlag: Elsevier Science

Buch, Englisch, 256 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 450 g

Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.

Autoren/Hrsg.

Hussain, Khursheed

Lone, Sameena Maqbool

Masoodi, Khalid Z.

Balkhi, Syed Minu

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Physik Angewandte Physik Biophysik

Inhaltsverzeichnis

1. Introduction to
2. Introduction to laboratory procedures
3. Preparation of solutions and buffers
4. Qualitative analysis of carbohydrates
5. Qualitative analysis of proteins
6. pH (negative logarithm of hydrogen ion concentration)
7. Spectrophotometry
8. Carbohydrates
9. Amino acids and proteins
10. Enzyme assays
11. Vitamin assays
12. Phenolics
13. Plant pigments
14. Plant
15. Antinutritional factors in plants
16. Toxins
17. Extraction and identification of polyacetylenes
18. Alkaloids
19. Plant hormones
20. Immunochemical methods
21. Nitrogenase activity
22. Chlorophyll
23. Proximate analysis
24. Minerals
25. Antinutritional factors in plants
26. Antioxidants
27. Lipids

Über Autor(innen)

Balkhi, Syed Minu
Dr. Syed Minu Balkhi holds a PhD in Biochemistry from the University of Kashmir, India. She qualified for the JRF-NET CSIR and obtained a fellowship during her PhD program in 2013. She is currently a Lecturer at Government Boys Higher Secondary School, Humhama, India. Prior to this she was a Post-Doctoral Associate at the Department of Immunology, University of Pittsburgh, USA. She has contributed to various publications.

Masoodi, Khalid Z.
Dr. Khalid Zaffar Masoodi is a young scientist who works as an Assistant Professor in the Division of Plant Biotechnology at the Sher-e Kashmir University of Agricultural Sciences and Technology in Kashmir (India). He holds a Master's degree in Biotechnology and a doctorate in Plant Biotechnology. Dr. Masoodi is the recipient of numerous awards, including the SERB Early Career Research Award 2016, the SBUR Travel Award in the United States of America in 2012, and the Society of Endocrinology Journal Award in 2018 at Glasgow United Kingdoms. Dr. Masoodi has a distinguished academic record, having qualified for the CSIR-UGC net in Life Sciences, the DBT-BET JRF, and the Graduate Aptitude Test in Engineering and Sciences (GATE) while doing his Masters in Biotechnology. Dr. Masoodi has filed Six patent applications in last 2 years, has over 12 years of teaching and research experience, and has published numerous scientific articles in the fields of cancer research, human disease research, and biotechnology research. His laboratory (Transcriptomics Laboratory, often known as K-Lab, after the Klenow fragment) at SKUAST-K is fully equipped with fluorescence imaging, real-time PCR, cell culture, and Western blotting including other state-of-the-art equipment. His research interests span multiple dimensions, including transcriptomics of plant stress biology (biotic/abiotic) and cancer biology. Apart from that, he is extensively involved in the research of new drugs from medicinal plants. Additionally, he is a co-author of Advanced Molecular Biology and Biotechnology Methods (Academic Press, 2020).

Hussain, Khursheed
Khursheed Hussain, has been serving as Assistant Professor at Sher-e-Kashmir University of Sciences & Technology in India for 13 years. He served as Scientific Officer to Vice Chancellor from 2015-2020. He has published more than 120 research articles, 25 review articles and many books. His contribution in the field of research is in varietal and hybrid development as Principal/Co-breeder of 7 vegetable varieties with other varieties in the pipeline. He has been an advisor of more than 50 PG students and has been invited to give many lectures and trainings.