Proteomic and Metabolomic Approaches to Biomarker Discovery

Erscheinungsjahr 2013
ISBN: 978-0-12-394446-7
Verlag: Elsevier Science Publishing Co Inc

Buch, Englisch, 488 Seiten, Format (B × H): 195 mm x 241 mm, Gewicht: 1209 g

Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes.

Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process.

A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research.

Zielgruppe

<p>Biochemists, analytical chemists, bioanalytical chemists, biologists, medical technicians, clinical technicians, researchers in pharmaceuticals, graduate students and all interested in the field of biomarkers research<b>. </p></b>

Autoren/Hrsg.

Issaq, Haleem J.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Pharmazeutische Technologie
• Naturwissenschaften Chemie Analytische Chemie
• Naturwissenschaften Chemie Organische Chemie Biochemie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie

Inhaltsverzeichnis

- Biomarker Discovery: Study Design and Execution
- Proteomic Technologies for Biomarker Discovery
- Sample Preparation for Proteomics
- Sample preparation For metabolomics
- SOP for serum and plasma
- Current NMR Strategies
- Data-independent Mass Spectrometry Extends Detectable Dynamic Range
- GC/MS
- LC/MS for: (a) Metabolomics
- CE/MS for (a) Proteomics (b) Metabolomics
- Gel electrophoresis
- The Role of Two-dimensional Gel Electrophoresis in Biomarker Discovery
- Affinity
- Asp selective Microwave-supported acid proteolysis
- Fractionation of complex mixtures
- Protein and Metabolite Identification
- Quantitative proteomics
- Quantitative analysis of metabolites in biological fluids
- Statistical & computational analysis
- Top-down Proteomics for Biomarker Discovery
- A role for protein-protein interaction networks in the identification and characterization of potential biomarkers
- Protein microarrays
- Autoantibodies as biomarkers
- MicroRNAs and Biomarker Discovery
- Tissue imaging for biomarker discovery
- MS approaches for biomarker verification
- MS metabolomic data handling for biomarker discovery
- Biomarker Validation

Über Autor(innen)

Issaq, Haleem J.
Haleem J. Issaq received his B.S. degree from the American College of Istanbul, Turkey, a M.S. degree from the Technion, Israel Institute of Technology, and a Ph.D. in analytical chemistry from Georgetown University in 1972. He was a Principal Scientist and Head of the Separation Technologies and Assay Development Group within the Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., located within the National Cancer Institute at Frederick, Maryland. His research interests include multidimensional separation of complex protein/peptide mixtures using liquid-phase-based separations (LC/LC, IEF/LC, and LC/CE), fast separation of estrogens, and use of HPLC/MS metabolomics for disease biomarker detection. He is the author or coauthor of over 350 scientific publications and the editor of A Century of Separation Science. He is the recipient of numerous awards, including Maryland Chemist of the Year.