Iyengar G Protein Pathways, Part A: Receptors

1. Auflage 2001
ISBN: 978-0-08-088422-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 343, 680 Seiten, Web PDF

Reihe: Methods in Enzymology

G Protein Pathways is the first of three volumes examining the nature of heterotrimeric G proteins. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.

Autoren/Hrsg.

Iyengar, Ravi

Inhaltsverzeichnis

1;Front Cover;1
2;G Protein Pathways;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 343;10
6;Preface;16
7;Volume in Series;20
8;Section I: G Protein-Coupled Receptors;40
8.1;A. Theoretical Evaluation of Receptor Function;40
8.1.1;Chapter 1. Considerations in the Evaluation of Inverse Agonism and Protean Agonism at GProtein-Coupled Receptors;42
8.1.2;Chapter 2. Theoretical Implications of Receptor Coupling to Multiple G Proteins Based on Analysis of Three-State Model;56
8.2;B. Design and Use of Receptor Ligands;40
8.2.1;Chapter 3. Use of Retinal Analogues for the Study of Visual Pigment Function;68
8.2.2;Chapter 4. Design and Synthesis of Peptide Antagonists and Inverse Agonists for G Protein-Coupled Receptors;88
8.2.3;Chapter 5. Design of Peptide Agonists;112
8.2.4;Chapter 6. Design of Nonpeptides from Peptide Ligands for Peptide Receptors;130
8.2.5;Chapter 7. Strategies for Mapping the Binding Site of the Serotonin 5-HT2A Receptor;162
8.3;C. Structural Characterization of Receptor Proteins;40
8.3.1;Chapter 8. Use of the Substituted Cysteine Accessibility Method to Study the Structure and Function of G Protein-Coupled Receptors;176
8.3.2;Chapter 9. Mass Spectrometric Analysis of GProtein-Coupled Receptors;196
8.3.3;Chapter 10. Probing the Higher Order Sructure of G Protein- Coupled Receptors Using Tethered Cleavage Methods;201
8.3.4;Chapter 11. Use of Fluorescence Spectroscopy to Study Conformational Changes in the ß2-Adrenoceptor;209
8.3.5;Chapter 12. Crystallization of Membrane Proteins in Cubo;222
8.3.6;Chapter 13. N-Linked Carbohydrates on G Protein-Coupled Receptors: Mapping Sites of Attachment and Determining Functional Roles;239
8.3.7;Chapter 14. Magic Angle Spinning Nuclear Magnetic Resonance of Isotopically Labeled Rhodopsin;251
8.3.8;Chapter 15. Use of Nuclear Magnetic Resonance to Study the Three-Dimensional Structure of Rhodopsin;262
8.4;D. Design and Use of Engineered Receptor Proteins;40
8.4.1;Chapter 16. Tools for Dissecting Signaling Pathways in Vivo: Receptors Activated Solely by Synthetic Ligands;271
8.4.2;Chapter 17. Analysis of Structure–Function from Expression of G Protein-Coupled Receptor Fragments;287
8.4.3;Chapter 18. Construction and Analysis of Function of G Protein-Coupled Receptor–G Protein Fusion Proteins;299
8.4.4;Chapter 19. Synthetic Gene Technology: Applications to Ancestral Gene Reconstruction and Structure Function Studies of Receptors;313
8.4.5;Chapter 20. Considerations in the Design and Use of Chimeric G Protein-Coupled Receptors ;334
8.5;E. Molecular Modeling Studies of Receptor Structure and Function;40
8.5.1;Chapter 21. Strategies for Modeling the Interactions of Transmembrane Helices of G Protein-Coupled Receptors by Geometric Complementarity Using the GRAMM Computer Algorithm;352
8.5.2;Chapter 22. Three-Dimensional Representations of G Protein- Coupled Receptor Structures and Mechanisms;368
8.6;F. Analysis of Receptor Protein Coupling;40
8.6.1;Chapter 23. Reconstitution of G Protein-Coupled Receptors with Recombinant GProteinaand ß. Subunits;411
8.6.2;Chapter 24. Cell-Free Membrane Desensitization Assay for G Protein-Coupled Receptors;433
8.6.3;Chapter 25. Methods to Determine the Constitutive Activity of Histamine H2 Receptors;444
8.6.4;Chapter 26. Expression of G Protein-Coupled Receptors and G Proteins in Sf9 Cells: Analysis of Coupling by Radioligand Binding;456
8.6.5;Chapter 27. G Protein-Coupled Receptors and Proliferative Signaling;469
8.7;G. Characterization of Receptor Heterogeneity;40
8.7.1;Chapter 28. Genetic Analysis of G Protein-Coupled Receptor Genes;487
8.7.2;Chapter 29. IdentiIfication of Adrenergic Receptor Polymorphisms;498
8.7.3;Chapter 30. Strategies and Requirements for the Detection of RNA Editing in G Protein Coupled-Receptor RNA;515
8.8;H. The Study of Receptor Trafficking;40
8.8.1;Chapter 31. Fluorescence MicroscopyTechniques for the Study of G Protein-Coupled Receptor Trafficking;531
8.8.2;Chapter 32. Measurement of Receptor Desensitization and Internalization in Intact Cells;545
8.8.3;Chapter 33. Morphological and Biochemical Strategies for Monitoring Trafficking of Epitope-Tagged G Protein-Coupled Receptors in Agonist- Naive and Agonist-Occupied States;569
9;Section II: Regulators of GPCR Function;584
9.1;A. G Protein-Coupled Receptor Kinases (GRKs);584
9.1.1;Chapter 34. Characterization of G Protein-Coupled Receptor Kinases;586
9.1.2;Chapter 35. Regulation of G Protein-Coupled Receptor Kinase 2;598
9.1.3;Chapter 36. Rhodopsin and Its Kinase;617
9.2;B. Arrestins and Novel Proteins;584
9.2.1;Chapter 37. Characterization of Arrestin Expression and Function;639
9.2.2;Chapter 38. Identification of Novel G Protein-Coupled Receptor-Interacting Proteins;650
10;Author Index;662
11;Subject Index;700