Intracellular pH and its Measurement

1. Auflage 1989
ISBN: 978-0-8493-4916-4
Verlag: CRC Press

Buch, Englisch, 192 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 567 g

This volume introduces a summary of all the techniques used to estimate pH reliably. Emphasis is placed on the techniques that provide the most reliable and detailed data. The role of cell pH is explained with special emphasis on enzymology and membrane transport and bioenergetics. This book was written especially for molecular biologists, biochemists and biophysicists.

Autoren/Hrsg.

Kotyk, Arnost

Slavik, Jan

Fachgebiete

• Naturwissenschaften Physik Physik Allgemein Geschichte der Physik
• Naturwissenschaften Physik Physik Allgemein Experimentalphysik
• Naturwissenschaften Physik Physik Allgemein Theoretische Physik, Mathematische Physik, Computerphysik

Inhaltsverzeichnis

The Concept of pH, Hydrolysis, and Buffers. pH in Biological Systems. Methods of Determining Intracellular pH with Electrodes. Assays of Intracellular pH Using Chemical Probes: Principles of pH Indicator Response. Assays of Intracellular pH Using Chemical Probes: Distribution Techniques. Assays of Intracellular pH Using Chemical Probes: Absorption Spectroscopy. Assays of Intracellular pH Using Chemical Probes: Fluorescence Spectroscopy. Assays of Intracellular pH Using Chemical Probes: Nuclear Magnetic Resonance Spectroscopy. The Choice of the Assay Method. Heterogeneity of Intracellular pH. Index.

Über Autor(innen)

Arnost Kotyk and Jan Slavik