Lipids and Membranes: Detection by Imaging and Lipidomics

Erscheinungsjahr 2026
ISBN: 978-0-443-43118-0
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm

Lipids are the cell’s hydrophobic metabolites, and their self-assembly into bilayers creates membranes that are critical barriers and organizing platforms in cells. Lipids are a chemically diverse set of biological molecules subject to rapid metabolism and transport, and this dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the Volumes on Lipids and Membranes provide a broad snapshot of the state of the art of methods for studying biological lipids and biomembranes. This part focuses on methods for lipid detection both by lipidomics-based mass spectrometry and by imaging, including both small-molecule and genetically encoded probes.

Autoren/Hrsg.

Weitere Mitwirkende

Baskin, Jeremy M.

Fachgebiete

• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Measurement of fatty acid esters of hydroxy fatty acids
Alan Saghatelian
2. Superresolution organelle imaging with lipophilic HIDE probes
Alanna Schepartz
3. Ultra-high resolution analysis of membrane lipids from 15N metabolically labeled mice
A. Shevchenko
4. Superresolution lipid imaging with expansion microscopy
Brittany White-Mathieu
5. High-resolution phosphoinositide analysis in healthy versus disease cells
Carsten Schultz
6. Alkyne lipids as tracers of lipid metabolism
Christoph Thiele
7. Imaging oxidative stress in biomembranes
Enver Izgu
8. Imaging the dynamics of phosphatidylinositol 4-phosphate metabolism
Fubito Nakatsu
9. Lipidomics for studying sphingolipids in cell death
G. Ekin Atilla-Gokcumen
10. Non-invasive imaging of phosphoinositides with genetically encoded lipid biosensors
Gerald Hammond
11. Profiling lipotypes at the single cell level in heterogenous populations
Giovanni D'Angelo
12. MS imaging of phosphoinositides
Laura Beth McIntire
13. Mass spectrometry based epilipidomics analysis
Maria Federova
14. Quantitative analysis of H-Ras localization using confocal microscopy
Mark Distefano
15. Metabolic labeling of glycerophospholipids
Michael Best
16. Lipid-protein interactions in membrane biology
Michal Grzybek
17. Imaging approaches to quantify protein dynamics within cellular membranes
Sarah Shelby
18. Lipid quantification in Niemann-Pick Disease
Stephanie M. Cologna

Über Autor(innen)

Baskin, Jeremy M.
Jeremy M. Baskin is Associate Professor, Nancy and Peter Meinig Family Investigator in the Life Sciences, and Director of the Chemistry-Biology Interface Program at Cornell University, with appointments in the Department of Chemistry and Chemical Biology and the Weill Institute for Cell and Molecular Biology. He was born and raised in Montreal, Canada and received his undergraduate education at the Massachusetts Institute of Technology, with a major in Chemistry and minors in Biology and Music. Jeremy carried out Ph.D. studies supported by NDSEG and NSF graduate fellowships in Carolyn Bertozzi's group at the University of California, Berkeley, focusing on development of bioorthogonal chemistries. Jeremy received postdoctoral training in lipid cell biology as a Jane Coffin Childs fellow at Yale University with Pietro De Camilli. Research in the Baskin lab centers on the chemical and cell biology of lipid metabolism and signaling. The Baskin lab exploits bioorthogonal chemistry to develop advanced tools for high-resolution lipid imaging and harnesses optogenetics and protein engineering for the design of membrane editors capable of spatiotemporal manipulation of the lipid composition of cellular membranes. Using these and other approaches, his lab elucidates novel mechanisms underlying physiological and pathological lipid metabolism and signaling events. Jeremy has been the recipient of numerous awards, including Beckman Young Investigator, Sloan Research Fellowship, NSF CAREER, ACS Young Academic Investigator, ASBMB Walter A. Shaw Young Investigator in Lipid Research, ICBS Young Chemical Biologist Award, and ACS Chemical Biology Young Investigator Award. He is currently Associate Editor at Biochemistry.