Volume 6
Buch, Englisch, 372 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 572 g
ISBN: 978-1-4615-7762-1
Verlag: Springer
More than ever, the introduction of new methods or techniques serves to stimulate progress into understanding the structure and function of the nervous system. This axiom is exemplified by recent techniques that have revolutionized several branches of neurochemistry and promise to remain dominant for many years. Such developments underscore the need to remain abreast of new research strategies and provide further justification for the present series. The use of high performance liquid chromatography combined with bioassay methods provides a powerful technique for iso lation and assay of trace amounts of neuropeptides. Two chapters in the present volume deal with this subject: one (Stenn and Lewis) describes the assay of enkephalins, and the other (Chretien and Seidah), on lipo tropic peptides, includes procedures for structural analysis by microse quencing. These methods rival earlier ones for peptide separations in speed, sensitivity, and cost and have general applicability in most labo ratories. High performance liquid chromatography has also largely sup planted earlier and more tedious procedures for the assay of catechola mines, as described in Chapter 7 by Causon. As in earlier volumes, we have striven to retain a balance between studies on intact tissues and those on subcellular components.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Section I Ultrastructure and Fragmentation of Neural Tissues.- 1 Techniques for Neurochemical Research on the Retina.- 2 Isolation of Cells from Frozen Brain Tissue and Storage of Isolated Cells in the Frozen State.- Section II Properties of Intact Neural Tissues.- 3 The Deoxyglucose Method for the Measurement of Local Glucose Utilization and the Metabolic Mapping of Functional Neural Pathways in the Central Nervous System.- 4 Continuous-Injection Methods for the Measurement of Flux across the Blood-Brain Barrier: The Steady-State, Initial-Rate Method.- Section III Components of Neural Tissues—Peptide Hormones and Amines.- 5 Methods for Isolation, Characterization, and Sequence Analysis of Enkephalin Precursors.- 6 Microsequence of Polypeptide Hormones: Its Usefulness to Monitor the Isolation of Novel Molecules.- 7 High-Performance Liquid Chromatographic Separation and Determination of Catecholamines.- Section IV Components of Neural Tissues—Enzymes and Proteins.- 8 Purification of Brain Carbonic Anhydrase by Preparative and Immunologic Techniques.- 9 Research Methods in Studies with the P2 Basic Protein.- 10 Methods for the Identification and Characterization of Glycoproteins in Central and Peripheral Myelin.
