A Laboratory Course in Tissue Engineering

1. Auflage 2017
ISBN: 978-1-138-40735-0
Verlag: Taylor & Francis Ltd

Buch, Englisch, 304 Seiten, Format (B × H): 156 mm x 234 mm, Gewicht: 454 g

Filling the need for a lab textbook in this rapidly growing field, A Laboratory Course in Tissue Engineering helps students develop hands-on experience. The book contains fifteen standalone experiments based on both classic tissue-engineering approaches and recent advances in the field. Experiments encompass a set of widely applicable techniques: cell culture, microscopy, histology, immunohistochemistry, mechanical testing, soft lithography, and common biochemical assays. In addition to teaching these specific techniques, the experiments emphasize engineering analysis, mathematical modeling, and statistical experimental design.

A Solid Foundation in Tissue Engineering and Communication Skills

Each experiment includes background information, learning objectives, an overview, safety notes, a list of materials, recipes, methods, pre- and postlab questions, and references. Emphasizing the importance for engineering students to develop strong communication skills, each experiment also contains a data analysis and reporting section that supplies a framework for succinctly documenting key results. A separate chapter provides guidelines for reporting results in the form of a technical report, journal article, extended abstract, abstract, or technical poster.

Customize Your Courses with More Than a Semester‘s Worth of Experiments

The book is a convenient source of instructional material appropriate for undergraduate or graduate students with fundamental knowledge of engineering and cell biology. All of the experiments have been extensively tested to improve the likelihood of successful data collection. In addition, to minimize lab costs, the experiments make extensive use of equipment commonly found in laboratories equipped for tissue culture. A solutions manual, available with qualifying course adoption, includes answers to pre- and postla

Autoren/Hrsg.

Micou, Melissa Kurtis

Fachgebiete

• Technische Wissenschaften Maschinenbau | Werkstoffkunde Technische Mechanik | Werkstoffkunde
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik

Inhaltsverzeichnis

Getting Started in the Lab. Lab Safety. Essential Lab Skills for Tissue Engineers. Isolation of Primary Chondrocytes from Bovine Articular Cartilage. Measuring and Modeling Growth of a Cell Population. Purification of a Cell Population Using Magnetic Cell Sorting. Decellularized Matrices for Tissue Engineering. Effect of Plating Density on Cell Adhesion to Varied Culture Matrices. Dynamic versus Static Seeding of Cells onto Biomaterial Scaffolds. Cell Patterning Using Microcontact Printing. Measuring and Modeling the Motility of a Cell Population Using an Under-Agarose Assay. Characterizing Matrix Remodeling Through Collagen Gel Contraction. Effect of Substrate Stiffness on Cell Differentiation. Effect of Culture Configuration (Two versus Three Dimensions) on Matrix Accumulation. Combining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell Fate. The Fahraeus-Lindqvist Effect: Using Microchannels to Observe Small Vessel Hemodynamics. Examining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration System. Contribution of Tissue Composition to Bone Material Properties. Technical Communication: Presenting Your Findings. Appendices. Index.

Über Autor(innen)

Melissa Kurtis Micou, Ph.D., is a lecturer in the Department of Bioengineering at the University of California, San Diego. She has taught tissue-engineering lecture and lab courses for undergraduate students for the past ten years. Dawn M. Kilkenny, Ph.D., is an assistant professor at the Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, and is academic advisor to the IBBME undergraduate teaching laboratory. Her research interests include cellular signaling, fluorescent protein technology, and microscopy.