Vibrational Spectroscopy in Protein Research

From Purified Proteins to Aggregates and Assemblies
Erscheinungsjahr 2020
ISBN: 978-0-12-818610-7
Verlag: Elsevier Science

Buch, Englisch, 608 Seiten, Format (B × H): 191 mm x 235 mm, Gewicht: 1250 g

Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies.

Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.

Zielgruppe

Active researchers and students in biochemistry, molecular biology, pharmaceutical science, cellular biology, applied microbiology, and biotechnology; medical chemists; clinician scientists

Autoren/Hrsg.

Ozaki, Yukihiro

Baranska, Malgorzata

Lednev, Igor K

Wood, Bayden R

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Chemie Analytische Chemie Massenspektrometrie, Spektroskopie, Spektrochemie

Inhaltsverzeichnis

1. ATR-FTIR Spectroscopy and Spectroscopic Imaging of Proteins 2. Light-Induced Difference FTIR Spectroscopy of Photoreceptive Proteins 3. Quantum Cascade Laser-based IR Transmission Spectroscopy of Proteins in Solution 4. Theoretical Simulation of Protein Two-Dimentional Infrared Spectroscopy 5. Infrared Spectroscopy and Imaging for Understanding Neurodegenerative Protein-Folding Diseases 6. Near-infrared (NIR) spectroscopy in protein research 7. Vibrational imaging of proteins: changes in the tissues and cells in the lifestyle disease studies 8. Interpretation of Vibrational Optical Activity Spectra of Proteins 9. Nano-scale analysis of protein self-assemblies 10. Vibrational Spectroscopic Analysis and Quantification of Proteins in Human Blood Plasma and Serum 11. Vibrational spectroscopy in protein research towards virus identification: challenges, new research and future perspectives 12. Two-Dimensional Correlation Spectroscopy of Proteins 13. Resonance Raman spectroscopy of hemoglobin in red blood cells 14. Ultraviolet Raman spectroscopy for understanding structure and formation mechanism of amyloid fibrils 15. Recent advances in Raman spectroscopy of proteins for disease diagnosis 16. Dynamics and allostery of human hemoglobin as elucidated by time-resolved resonance Raman spectroscopy 17. Immuno-SERS: from nanotag design to assays and microscopy 18. Highly localized characterization of protein structure and interaction by surface-enhanced Raman scattering 19. Surface-enhanced Raman scattering (SERS) chemosensing of proteins

Über Autor(innen)

Lednev, Igor K
Dr. Igor Lednev received his PhD degree from The Moscow Institute of Physics and Technology, Russian Federation before joining the University of Albany as faculty. As an academic visitor, he worked in several leading laboratories around the world including the United Kingdom, Japan, Canada, and Germany. Dr. Lednev's current research is focused on the development and application of novel laser spectroscopy for biomedical and forensic applications.

Wood, Bayden R
Dr. Bayden R. Wood is an Australian Research Council Future Fellow working at the Centre for Biospectroscopy located in the School of Chemistry, Monash University, Malborne, Australia. His research is concerned primarily with vibrational spectroscopy of tissues, cells, and biomolecules, and monitoring home aggregation in single living red blood cells associated with erythrocyte disorders, including malaria and sickle cell disease, using resonance Raman spectroscopy.

Baranska, Malgorzata
Dr. Malgorzata Baranska leads the Raman Imaging Group at The Jagiellonian University, Kraków, Poland. In her research, Dr. Baranska focuses on analyzing bioactive compounds using spectroscopic methods, particularly modern Raman techniques. The main direction of her research is related to development treatments for life style diseases, including new drugs of endothelium bioactivity.

Ozaki, Yukihiro
Dr. Ozaki received his B.Sc. in Chemistry in 1973 and PhD in Physical and Inorganic Chemistry in 1978, both from Osaka University. After over two years at the National Research Council of Canada as a research associate, he joined Jikei University School of Medicine in Tokyo. In 1989, he became associate professor and later full professor in the Chemistry Department at Kwansei Gakuin University until March 2018. He was a Vice President of Kwansei Gakuin University from April 2013 to March 2018. Currently Professor Ozaki is a professor emeritus of Kwansei Gakuin University. Ozaki has received many awards, including the 1998 Tomas Hirschfeld Award, 2001 EAS Award for Achievements in Near Infrared Spectroscopy, 2002 Spectroscopical Society of Japan Award, 2005 Science and Technology Award of Japanese Government, 2008 Japan Society for Analytical Chemistry Award, 2014 Bomem-Michelson Award, 2017 Chemical Society of Japan Award, and the 2018 Medal with Purple Ribbon.