Pandey / Sharma / Shanmugam GTPases
2015
ISBN: 978-3-319-11611-2
Verlag: Springer International Publishing
Format: PDF
Kopierschutz: 1 - PDF Watermark
Versatile Regulators of Signal Transduction in Plants
E-Book, Englisch, 81 Seiten, eBook
Reihe: SpringerBriefs in Plant Science
ISBN: 978-3-319-11611-2
Verlag: Springer International Publishing
Format: PDF
Kopierschutz: 1 - PDF Watermark
G proteins are the key regulators for a wide range of cellular processes in animals and plants. In comparison to animals and yeast, plants have a single Rho-GTPase subfamily called Rho-like GTPases (ROPs). The ROP family of monomeric GTPases has emerged as a versatile and key regulator in plant signal transduction processes. During the past few years’ studies on plant RHO-type (ROP) GTPase have generated new insights into their role in diverse processes ranging from cytoskeletal organization, polar growth, development to stress and hormonal responses. Studies have shown that plants have evolved specific regulators and effector molecules. ROP GTPases possess the ability to interact with these multiple regulator and effector molecules that ultimately determines their signaling specificity. Recently, genome wide studies in plants have shown that the Arabidopsis genome encodes 93, and rice has nearly 85 small GTPase homologs. And we have been able to identify four new homologs in the rice genome. Here, we focus on the complete phylogenetic, domain, structural and expression analysis during stress and various developmental processes of small GTPases in plants. The comparison of gene expression patterns of the individual members of the GTPase family may help to reveal potential plant specific signaling mechanisms and their relevance. Also, we are summarizing the role of currently known ROP GTPases and their interacting proteins with brief description, simultaneously, comparing their expression pattern based on microarray data. Overall, we will be discussing the functional genomic perspective of plant Rho like GTPases and their role in regulating several physiological processes such as stress, hormonal, pollen tube, root hair-growth and other developmental responses.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
1. Overview of G proteins (GTP binding proteins) in Eukaryotes1.1 Overview1.2 Small G-protein structure and Domain features1.3 Biochemical regulation of small GTPases1.4 GEFs1.5 GAPs1.6 GDIs1.7 Localization and Post-translational modifications References 2. Overview of Small GTPase Signaling Proteins in Plants2.1 Abstract2.2 Introduction 2.3 Small GTPase Complement in Plantae2.4 Plant Specific Functions of ROPsReferences 3. Identification and Classification of Rho GTPases in Plants3.1 Introduction3.2 Nomenclature3.3 ROP GTPase complement in Arabidopsis3.4 ROP GTPase complement in riceReferences 4. Sequence, Structure, and Domain Analysis of GTPases in Plants 4.1 Introduction4.2 Domain Identification and Confirmation of Rice Small GTPases4.3 Multiple alignment and phylogenetic analysis4.4 Comparative Phyletic analysis of Rice Small GTPase genes4.5 Gene nomenclature and localization of small GTPases4.6 G-Domain Conservation PatternReferences 5. Expression of Small GTPases Under Stress and Developmental Conditions in Plants 5.1 Expression Pattern of OsGTPases in Abiotic Stress 5.2 Expression Pattern of OsGTPases in Developmental Stages5.3 Expression Pattern of OsGTPases during Phytohormone treatment5.4 Expression Pattern of AtGTPases in Devlopmental stages 5.5 Expression Pattern of AtGTPases in Abiotic Stress 5.6 Expression Pattern of AtGTPases during Phytohormone treatment References 6. Emerging Roles of Rho GTPases in Plants 6.1 Rho GTPases: Versatile signaling molecules in plants6.2 Rho Activates plant defense mechanisms6.3 Role in intracellular trafficking and cell polarity6.4 Role in pollen tube growth6.5 Role in root hair development6.6 Small GTPases Controls Cell MorphogenesisReferences 7. Cellular Localization of Small GTPases 7.1 Membrane association of RAC/ROP GTPase7.2 Hypervariable Region regulate RAC/ROP localization7.3 Post-translational Lipid Modifications determines the ROP Activity7.4 Significance of Subcellular localization in ROP SignalingReferences 8. Functional Genomic Perspective of Small GTPases 8.1 Regulatory mechanism of Rho signaling8.1.1 Regulators and Effectors of ROP8.1.2 ROP interactive CRIB domain containing proteins (RICs)8.1.3 Two counteractive pathways coordinate the actin dynamics in pollen tube elongation8.1.4 The antagonistic ROP2/RIC1 pathway promote microtubule organization 8.2 Unconventional effectors of ROP/RAC pathwaysReferences9. Systemic Approaches to Resolve Spatio-Temporal Regulation of GTPase Signaling 9.1 Introduction9.2 Detection of Rho GTPase activity in plant cells9.3 FRET (fluorescence resonance energy transfer) assay9.4 Biochemical assay for ROP activity detection9.5 Light-gated protein interaction9.6 Significance of Systemic Approaches to Measure Signaling ModularityReferences 10. Key Questions and Future Prospects 10.1 Introduction10.2 GTPase and lipid interaction10.3 Upstream and downstream regulators10.4 Future perspectivesReferences
