Buch, Englisch, Band 2862, 365 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 908 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2862, 365 Seiten, Book w. online files / update, Format (B × H): 183 mm x 260 mm, Gewicht: 908 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-4167-5
Verlag: Springer US
This detailed volume explores techniques utilizing the fission yeast , a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and more. The volume also includes chapters with a focus on computational methods specific to the approaches. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, serves as the ideal guide for researchers in the fission yeast community working with emerging technologies.
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Weitere Infos & Material
Dual Luciferase Reporter Assay in Schizosaccharomyces pombe.- High-Throughput Measurement of Single Fission Yeast Cell Volume Using Fluorescence Exclusion.- Measuring Cell Dimensions in Fission Yeast Using Machine Learning.- Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast S. pombe.- Live Imaging of Fission Yeast Single Cell Lineages Using a Microfluidic Device.- Analysis of Cell Wall Mechanics in Fission Yeast.- Pil1 Co-Tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe.- Bi-Molecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast.- Mapping Active RNA Polymerases in Proliferating and Quiescent Fission Yeast Cells Using Precision Run-On Sequencing.- Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection.- CRISPR–Cas9 Genome Editing in Auxotrophic and Non-Auxotrophic Fission Yeast Strains.- Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast.- SIMPLE Method for Plasmid Editing.- Investigating Fission Yeast Mutagenesis Using Canavanine Sensitivity Assays.- A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation.- Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.- Assessing Drug Sensitivity in Fission Yeast Using Half-Maximal Inhibitory Concentration (IC50) Assays.- ChIPmentation for Epigenomic Analysis in Fission Yeast.- Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays.- Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval.- Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.- Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag.- Identifying Drug Sensitivities in Fission Yeast by Assessing Growth Kinetics.- An “In Schizo” Evaluation System to Screen for Human Kinesin-5 Inhibitors.- Automated Machine Learning Tools to Build Regression Models for Schizosaccharomyces pombe Omics Data.
