Animal Cell Biotechnology

Methods and Protocols
Fourth Auflage 2020
ISBN: 978-1-0716-0190-7
Verlag: Springer US

Buch, Englisch, Band 2095, 399 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 976 g

Reihe: Methods in Molecular Biology

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Pörtner, Ralf

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Pharmazie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

Preface…Table of Contents…Contributing Authors…
Part I Cell Line and Media Development
1 Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation Johannes Möller, Katrin Korte, Ralf Pörtner, An-Ping Zeng, and Uwe Jandt
2 Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays Oliver Wehmeier and Alexander Loa
3 Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment Patrick Mayrhofer and Renate Kunert
Part II Techniques for Process Development
4 ambr® 15 Microbioreactors for CHO Cell Cultivation Steve R.C. Warr 
5 Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications Vincent Wiegmann, Cristina Bernal Martinez, and Frank Baganz 6 HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR)Tibor Anderlei, Mike Keebler, Jordi Joan Cairó, and Martí Lecina
7 Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode  Tim Bürgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl, and Tibor Anderlei
8 Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors Moritz Wolf and Massimo Morbidelli
9 Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production Alexander Nikolay, Thomas Bissinger, Gwendal Gränicher, Yixiao Wu, Yvonne Genzel, and Udo Reichl
10 How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale Cedric Schirmer, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl, and Dieter Eibl
Part III Model-Based Techniques for Process Development
11 Generic Workflow for the Setup of Mechanistic Process Models Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, and Christoph Herwig
12 Estimation of Process Model Parameters Sahar Deppe, Björn Frahm, Volker C. Hass, Tanja Hernández Rodríguez, Kim B. Kuchemüller, Johannes Möller, and Ralf Pörtner
13 Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments Kim B. Kuchemüller, Ralf Pörtner, and Johannes Möller
14 Design, Optimization, and Adaptive Control of Cell Culture Seed Trains Tanja Hernández Rodríguez and Björn Frahm
Part IV Process Analysis
15 High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays  Sebastian Giehring 16 Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture Olivier Henry, Eric Karengeraa, Florian Cambay, and Gregory De Crescenzo
17 Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells Julia Hennicke and Renate Kunert
18 Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples Hesham K. Yosef and Karin Schütze 
19 An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures Mario Lederle, Mircea Tric, Claudio Packi, Tobias Werner, and Philipp Wiedemann
20 Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures Lukas Käßer, Jan Zitzmann, Tanja Grein, Tobias Weidner, Denise Salzig, and Peter Czermak
Part V Downstream Techniques 21 Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method Ricardo J.S. Silva, João Mendes, Manuel J.T. Carrondo, Paula M. Marques, and Cristina Peixoto 22 Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium- Dependent Fragment Complementation System Gavin McGauran, Sara Linse, and David J. O’Connell