Ramos Comprehensive two dimensional gas chromatography

1. Auflage 2009
ISBN: 978-0-08-093269-9
Verlag: Elsevier Science & Technology
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, 324 Seiten, Format (B × H): 165 mm x 240 mm

The book will review the basic concepts and highlight the most relevant advances and developments that have taken place in the field of comprehensive two dimensional gas chromatography (GC x GC) since its introduction in 1991. The several instrumental and technical approaches assayed and developed during these fifteen years and that have contributed to the development of this powerful separation technique and to its increasing application in many application areas will be explained and comprehensively illustrated through a number of chapters devoted these specific topics. More specialized aspects of the technique, including theoretical aspects, modelization of the chromatographic process, software developments, and alternative couplings will also be covered. Finally, special attention will be paid to data treatment, for both qualitative and quantitative analysis. This book will be a practical resource that will explain from basic to specialized concepts of GC x GC and will show the current state-of-the-art and discuss future trends of this technique.

The book will contain 12 chapters written by different experts in each particular topic or application field and will be organized in two sections. Part I will cover the principles of the technique, its basic concepts and instrumentation, theoretical aspects, and some specialized topics devoted to data analysis, last developments and future trends. After an introductory chapter, in which the concept of ¡§multidimensionality¡? in gas chromatography will be discussed and the key issues, problems and challenges in GC x GC presented, the basic instrumentation for GC x GC will be described. The description of the several constituents of the basic GC x GC instrument will provide the reader the required understanding about the nature of the separation process in GC x GC and the relevance and potential influence of the different parts of the instrument in the final separation and detection of the analytes. The basic requirements of the GC columns, the different possible analytical approaches and the basic criteria for column selection will be explained. This chapter will also review the several models and types of modulators developed to interface the two GC columns involved in the separation process, highlighting their relative merits. The basic requirement for the detectors to be used in combination with GC ?e GC will be explained and the main advantages, limitations and respective application fields of the most frequently used ones revised.

The basic theory behind the GC x GC process is not evident and because of the novelty of the technique, it has not been until recently that the practitioners agreed about the nomenclature to be used to define the chromatographic concepts involved in the process. The more relevant aspects in this field will be covered in a specific chapter about theoretical aspects. The several attempts to model the GC x GC process on the basis of present knowledge will be summarized and future demands in this particular field stated.

The counterpoint to the high separation power of GC x GC is the complexity of the resulting chromatograms and the associated data file, peak list and quantitative data. The treatment of the high density GC x GC data set requires sophisticated algorithms to generate the characteristic two- (and three-)dimensional chromatograms associated to this technique. However, the simple eye inspection of these contour plots is frequently not enough to retrieve the desired information and the use of chemometry based approaches is often mandatory. The same applies for the quantitative analytical aspects where special attention will be paid to quality assurance and quality control of the GC ?e GC data. Recent achievements in these fields will be summarized in two chapters devoted to data acquisition and data treatment, and chemometric approaches, respectively.

The last chapter in this part of the book will focus on recently introduced alt

Zielgruppe

The book will be addressed to serve as general reference for post-graduate students, as well as a practical reference guide for a wide range of researchers: chemist, biologists, biochemists, food chemists, microbiologists and everybody who need to learn about or use this separation technique for analytical purposes. Each chapter will contain enough references to the currently available literature to help as an effective source for more detailed information.

Autoren/Hrsg.

Ramos, Lourdes

Fachgebiete

• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie

Inhaltsverzeichnis

I. Principles, basic concepts and instrumentation
- Multidimensionality in gas chromatography: General concepts
o Basic concepts about multidimensionality
o Comprehensive two dimensional vs multidimensional gas chromatography
o GC x GC vs other chromatographic methods: Advantages and limitations
o Basic requirements and set-up for GC x GC
o References
- Basic instrumentation for GC x GC
o Columns and ovens
o Modulators
o Detectors
o Detecting problems: some relevant experimental aspects
o References
- Theoretical aspects
o Basic concepts and definition of chromatographic terms in GC ?e GC
o Nomenclature and conventions
o Modelization and prediction of the chromatographic separation
o References
- Data acquisition and data treatment
o Mathematical algorithms
o Qualitative data analysis: the contour plot
o Quantitative data analysis and background correction
o QA/QC
o References
- Chemometric approaches
o Chemometric tools for GC x GC data analysis
o Alternative procedures for data treatment
o Models
o Quantification
o References
- Alternative couplings involving GC x GC
o Off-line and on-line coupling of LC with GC x GC
o Other couplings
o References

II. Application areas
- Petrochemistry
- Air and aerosols
- Volatile compounds, essential oils and fragrances
o Plant analysis
o Cosmetics, fragrances and allergens
o Analysis of chiral compounds
- Food analysis
o Volatile components and flavors
o Less volatile food components
- Environmental applications:

o POPs and close related compounds
o Pesticides
o Analysis of chiral compounds
- Bioanalysis
o Analysis of drugs
o Metabolomics and other -omics