Ruso / Piñeiro Proteins in Solution and at Interfaces

Methods and Applications in Biotechnology and Materials Science
1. Auflage 2013
ISBN: 978-1-118-52317-9
Verlag: John Wiley & Sons
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 500 Seiten, E-Book

Reihe: Wiley Series on Surface and Interfacial Chemistry (NY)

Explores new applications emerging from our latestunderstanding of proteins in solution and at interfaces
Proteins in solution and at interfaces increasingly serve as thestarting point for exciting new applications, from biomimeticmaterials to nanoparticle patterning. This book surveys the stateof the science in the field, offering investigators a currentunderstanding of the characteristics of proteins in solution and atinterfaces as well as the techniques used to study thesecharacteristics. Moreover, the authors explore many of the new andemerging applications that have resulted from the most recentstudies. Topics include protein and protein aggregate structure;computational and experimental techniques to study proteinstructure, aggregation, and adsorption; proteins in non-standardconditions; and applications in biotechnology.
Proteins in Solution and at Interfaces is divided intotwo parts:
* Part One introduces concepts as well as theoretical andexperimental techniques that are used to study protein systems,including X-ray crystallography, nuclear magnetic resonance, smallangle scattering, and spectroscopic methods
* Part Two examines current and emerging applications,including nanomaterials, natural fibrous proteins, and biomolecularthermodynamics
The book's twenty-three chapters have been contributed byleading experts in the field. These contributions are based on athorough review of the latest peer-reviewed findings as well as theauthors' own research experience. Chapters begin with a discussionof core concepts and then gradually build in complexity, concludingwith a forecast of future developments.
Readers will not only gain a current understanding of proteinsin solution and at interfaces, but also will discover howtheoretical and technical developments in the field can betranslated into new applications in material design, geneticengineering, personalized medicine, drug delivery, biosensors, andbiotechnology.

Autoren/Hrsg.

Ruso, Juan M.

Piñeiro, Ángel

Inhaltsverzeichnis

PREFACE ix
CONTRIBUTORS xiii
PART I
1 X-Ray Crystallography of Biological Macromolecules:Fundamentals and Applications 3
Antonio L. Llamas-Saiz and Mark J. van Raaij
2 Nuclear Magnetic Resonance Methods for Studying Soluble,Fibrous, and Membrane-Embedded Proteins 23
Victoria A. Higman
3 Small-Angle X-Ray Scattering Applied to Proteins in Solution49
Leandro Ramos Souza Barbosa, Francesco Spinozzi, Paolo Mariani,and Rosangela Itri
4 Analyzing the Solution State of Protein Structure,Interactions, and Ligands by Spectroscopic Methods 73
Veronica I. Dodero and Paula V. Messina
5 Resolving Membrane-Bound Protein Orientation and Conformationby Neutron Reflectivity 99
Hirsh Nanda
6 Investigating Protein Interactions at Solid Surfaces--InSitu, Nonlabeling Techniques 113
Olof Svensson, Javier Sotres, and Alejandro Barrantes
7 Calorimetric Methods to Characterize the Forces DrivingMacromolecular Association and Folding Processes 139
Conceic¸ ~ao A.S.A. Minetti, Peter L. Privalov, andDavid P. Remeta
8 Virtual Ligand Screening Against Comparative Models ofProteins 179
Hao Fan
9 Atomistic and Coarse-Grained Molecular Dynamics Simulations ofMembrane Proteins 193
Thomas J. Piggot, Peter J. Bond, and Syma Khalid
PART II
10 Preparation of Nanomaterials Based on Peptides and Proteins209
Yujing Sun and Zhuang Li
11 Natural Fibrous Proteins: Structural Analysis, Assembly, andApplications 219
Mark J. van Raaij and Anna Mitraki
12 Amyloid-Like Fibrils: Origin, Structure, Properties, andPotential Technological Applications 233
Pablo Taboada, Silvia Barbosa, Josue Juarez, Manuel-AlatorreMeda, and Vyctor Mosquera
13 Proteins and Peptides in Biomimetic Polymeric Membranes283
Alfredo Gonzalez-Perez
14 Study of Proteins and Peptides at Interfaces By MolecularDynamics Simulation Techniques 291
David Poger and Alan E. Mark
15 A Single-Molecule Approach to Explore the Role of the SolventEnvironment in Protein Folding 315
Katarzyna Tych and Lorna Dougan
16 Enhanced Functionality of Peroxidases By Its Immobilizationat The Solid-Liquid Interface of Mesoporous Materials andNanoparticles 335
Jose Campos-Teran, Iker Inarritu, Jorge Aburto, and EduardoTorres
17 Superactivity of Enzymes in Supramolecular Hydrogels353
Ye Zhang and Bing Xu
18 Surfactant Proteins and Natural Biofoams 365
Malcolm W. Kennedy and Alan Cooper
19 Promiscuous Enzymes 379
Luis F. Olguin
20 Thermodynamics and Kinetics of Mixed Protein/SurfactantAdsorption Layers at Liquid Interfaces 389
Reinhard Miller, E.V. Aksenenko, V.S. Alahverdjieva, V.B.Fainerman, C.S. Kotsmar, J. Kragel, M.E. Leser, J.Maldonado-Valderrama, V. Pradines, C. Stefaniu, A. Stocco, and R.Wustneck
21 Application of Force Spectroscopy Methods to the Study ofBiomaterials 429
Chuan Xu and Erika F. Merschrod S.
22 Protein Gel Rheology 437
Katie Weigandt and Danilo Pozzo
23 Exploring Biomolecular Thermodynamics in Aqueous andNonaqueous Environments using Time-Resolved Photothermal Methods449
Randy W. Larsen, Carissa M. Vetromile, William A. Maza, KhoaPham, Jaroslava Miksovska
INDEX 473

Über Autor(innen)

JUAN M. RUSO is currently Associate Professor in theDepartment of Applied Physics at the University of Santiago deCompostela, Spain. He has contributed to more than 100 publicationson a broad range of physical, chemical, and biophysics studies. Hisresearch interests include protein ligand interactions, thermalstabilization of protein, Phase behavior, and self-assemblyprocesses in soft matter systems, biocompatible materials design,and nanocarrier design for targeted drug delivery.
ÁNGEL PIÑEIRO is an IPP Research Fellow at theDepartment of Applied Physics of the University of Santiago deCompostela, Spain. His current research interests include thedesign and characterization of self-assembled systems as well asthe study of macromolecules in solution, embedded in membranes orat interfaces. His work is mainly based on computational methodsincluding multiscale molecular dynamics simulations and thedevelopment of software for the analysis of different experimentalproperties.