Sandoval Lipases and Phospholipases

Methods and Protocols
2012
ISBN: 978-1-61779-600-5
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 861, 547 Seiten, eBook

Reihe: Methods in Molecular Biology

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Sandoval, Georgina

Inhaltsverzeichnis

Part I: Overview of Lipases, Phospholipases and Related Enzymes1. Lipases: An Overview Leticia Casas Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval and Alain Marty2. Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification Yassine Ben Ali, Robert Verger, and Abdelkarim Abousalham3. Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus Mónica Sánchez-González, Allan Blanco-Gámez, Roberto Parra-Saldívar, Juan Carlos Mateos-Díaz and Maria Isabel Estrada Alvarado4. Phospholipases: An Overview Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri and Michael H. GelbPart II: Screening and Sources of Lipases, Phospholipases and Its Inhibitors5. High-Throughput Sceening Method for Lipases/Esterases Eduardo Mateos-Díaz, Jorge Alberto Rodríguez, María de los Ángeles Camacho-Ruiz, Juan Carlos Mateos-Díaz6. Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries Dolores Reyes-Duarte, Manuel Ferrer, and Humberto García-Arellano7. Plant Lipases:Partial Purification of C. papaya LipaseIvanna Rivera, Juan Carlos Mateos-Díaz, and Georgina Sandoval 8. Plant Phospholipases: an OverviewGeliang Wang, Stephen Ryu, and Xuemin Wang 9. Lipase and Phospholipase Inhibitors: Design and ApplicationsMark E. Venable10. High-Throughput Assay of Secreted Phospholipases A2 Inhibitors Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing and Michael H. GelbPart III Cloning, Production, Purification and Characterization of Lipases and Phospholipases 11. Heterologous Expression Systems for Lipases: A ReviewFrancisco Valero12. Streptomyces Phospholipase D: Cloning and ProductionYozo Nakazawa13. Cloning of PLD2 from Baculovirus for Studies in Inflammatory ResponsesJulian Gomez-Cambronero and Karen M. Henkels 14. Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in PerliteSusana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres 15. Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27Pablo Fuciños, Ramón González, Estrella Atanes, Ana Belem Fernández Sestelo, Nelson Pérez-Guerra, Lorenzo Pastrana and María Luisa Rúa 16. Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase Habib Horchani, Ahmed Fendri, Hanen Louatia, Adel Sayari, Youssef Gargouri and Robert Verger 17. Phospholipase A2 Purification and Characterization; A Case StudyAida Karray, Youssef Gargouri, Robert Verger and Sofiane Bezzine Part IV: Optimization of the Biocatalyst and Reaction Conditions 18. The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of LipasesSophie Duquesne, Florence Bordes, Franck Fudalej, Jean-Marc Nicaud and Alain Martya 19. Molecular Modeling of Lipase Binding to a Substrate-Water InterfaceChristian C. Gruber and Jürgen Pleiss 20. Different Strategies for Hyperactivation of Lipase BiocatalystsJose M. Palomo and Jose M. Guisan 21. Rational Design of Immobilized Lipases and PhospholipasesAlberto del Monte Martínez and Bessy V. Cutiño Avila 22. Thermodynamical Methods for the Optimization of Lipase Catalyzed ReactionsEdmundo Castillo, Alejandro Torres-Gavilán, Georgina Sandoval , Alain MartyPart V: Applications of Lipases and Phopholipases 23. Lipases as Biocatalysts for the Synthesis of Structured Lipids Ram Chandra Reddy Jala, Peng Hu, Tiankui Yang, Yuanrong Jiang, Yan Zheng, Xuebing Xu24. Lipases-Catalyzed Modification of Phenolic Antioxidants Pamela Torres, Dolores Reyes-Duarte, Antonio Ballesteros and Francisco J. Plou25. Lipases as Catalysts in Synthesis of Fine Chemicals Alicia Baldessari26. Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides Alicia Baldessari and Luis E. Iglesias27. Lipases as Biocatalyst for Biodiesel Production Xiaohu Fan, Xochitl Niheus, Georgina Sandoval28. Biopolymer Synthesis Catalyzed by Lipases Karla A. Barrera-Rivera, Arturo Flores-Carreón, Antonio Martínez-Richa29. Phospholipases in Food Industry-A Review Víctor Casado, Diana Martín, Carlos Torres, Guillermo Reglero30. Lipase and Phospholipase Biosensors: A Review Enrique J. Herrera-López