Integrated Methods in Protein Biochemistry: Part C

Erscheinungsjahr 2023
ISBN: 978-0-443-18592-2
Verlag: Elsevier Science & Technology

Buch, Englisch, 412 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 930 g

Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ?? subunit and PLC-? enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.

Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more.

Zielgruppe

<p>Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists</p>

Autoren/Hrsg.

Shukla, Arun K.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. NanoBiT-based methods to monitor the activation and modulation of RTKs

2. The interplay of G-protein ?? subunit and PLC-? enzyme in PIP2 hydrolysis and downstream signaling

3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems

4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase

5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase

6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms

7. Covalent protein painting: MS-based protein footprinting

8. Characterization of GPCR signaling complexes using negative-staining electron microscopy

9. Probing protein misfolding and dissociation with free electron laser

10. Optimized protocol for the characterization of Cas12a activities

11. Proximity proteomics for the identification and characterization of extracellular vesicles

12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs)

13. Characterization of RRE domain in RiPP biosynthesis

14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations

15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging

16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry

17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins

18. Quantitative cysteinome profiling with DIA-ABPP

19. High-throughput screening and evolution of carbohydrate-active enzymes

20. Regulation of enzymes with light using unnatural amino acids

21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies

22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics

23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors

24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules

25. Reconstitution of the MLL1 complex and probing methyltransferase activity

26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy

27. Structural and functional analysis of mono-copper enzymes from fungal species

28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins.

29. Electrophysiology of ion-transporting rhodopsins

30. Measuring protein post-translational modifications using the protein semi-synthesis

31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2)

32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP)

33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry

34. Global substrate profiling of post-translational modifying enzymes

35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide

Über Autor(innen)

Shukla, Arun K.
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla's research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.