Buch, Englisch, 1259 Seiten, Format (B × H): 186 mm x 276 mm, Gewicht: 2866 g
Buch, Englisch, 1259 Seiten, Format (B × H): 186 mm x 276 mm, Gewicht: 2866 g
Reihe: Metabolic Pathway Engineering Handbook
ISBN: 978-0-8493-3923-3
Verlag: Taylor & Francis Inc
Christina Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.
Fundamentals
The first volume provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus subtilis, Streptomyces, filamentous fungi, annd mammalian cells using cell culture.
Tool and Applications
The second volume delves into evolutionary tools, including those associated with gene expression for metabolic pathway engineering. It covers applications of emerging technologies, including research on genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. It also evaluates emerging applications for producing fine chemicals, drugs, and alternative fuels.
Zielgruppe
Scientists and researchers, graduate students, and industrial users in metabolic and chemical engineering.
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Volume I
Introduction and Preface to
Handbook,Christina D. Smolke
Progression of biological
synthesis methods towards commercial relevance
The field of metabolic
engineering
An overview of the metabolic
pathway engineering handbook
Metabolic engineering: looking
towards the future
Section I.
Cellular Metabolism,Vincent Martin
Transport processes inside the
cell
Energy generating reactions
Biosynthesis of important
building blocks
Polymerization of building
blocks to macromolecules
Rare metabolic conversions
harvesting diversity through nature
Section II.
Balances and Reaction Models,Walter van Gulik
Growth nutrients and diversity
Mass balances, rates, and
experiments
Data reconciliation and error
detection
Black box models for growth and
product formation
Metabolic models for growth and
product formation
A thermodynamic description of
microbial growth
Section III.
Regulation of Metabolic Pathways,James Liao
Regulation of specific pathways
Regulating of secondary
metabolism
Building networks as assemblies of simpler control
schemes
Section IV.
Modeling Tools for Metabolic Engineering,Costas Maranas
Metabolic flux analysis
Metabolic control analysis
Structure and flux analysis of
metabolic networks
Constraint-based genome-scale
models of cellular metabolism
Multi-scale modeling of
metabolic regulation
Validation of metabolic models
Section V.
Developing Appropriate Hosts for Metabolic Engineering,Jens Nielsen
Escherichia coli as a well-developed host for metabolic engineering
Metabolic engineering in yeast
Metabolic engineering in Bacillus subtilis
Metabolic engineering in Streptomyces
Metabolic engineering in
filamentous fungi
Metabolic engineering in
mammalian cells using cell culture
Volume II
Section VI.
Evolutionary Tools in Metabolic Engineering,Claudia Schmidt-Dannert
Evolutionary engineering of
industrially important microbial phenotypes
Expanding enzyme function and
activity through molecular evolution
Evolving the function of DNA and
RNA regulatory regions through molecular evolution
Evolving pathways and genomes
for the production of natural and novel compounds
Models predicting optimized
evolutionary strategies
Section VII.
Gene Expression Tools for Metabolic Pathway Engineering,Christina Smolke
Low-copy number plasmids as
artificial chromosomes
Chromosomal engineering
strategies
Regulating gene expression
through engineered RNA technologies
Tools designed to regulate
translational efficiency
Regulating gene expression
through post-translational modifications
Engineering bifunctional enzymes
for optimized metabolite transfer between sequential conversion steps
Practical pathway engineering
demonstration in integrating tools
Section VIII.
Applications of Emerging Technologies to Metabolic Engineering,Jay Keasling
Genome-wide technologies: DNA
and phenotypic microarrays, proteomics
Monitoring and measuring the
metabolome
Section IX.
Future Prospects in Metabolic Engineering,Bernhard Palsson and Sang Yup Lee
Genome-scale models, systems
biology, and metabolic engineering
Cell-free systems for metabolic
engineering
Dynamic in silico cell models
Appendix I:
Tools for Experimentally Determining Flux through Pathways,Ralf Takors
Measuring flux by isotope
labeling
Tools for measuring intermediate
and product formation
Quantitative
analysis of labeling data
Appendix II: Applications,Brian
Pfleger and Brian Brazeau
Cofactor regulation in biocatalytic redox reactions
Metabolic engineering for fine chemical production
Metabolic engineering for drug production
Metabolic engineering for alternative fuels
