Spielman / Brand | Experimental Cell Biology of Taste and Olfaction | Buch | 978-0-8493-7645-0 | sack.de

Buch, Englisch, 464 Seiten, Format (B × H): 163 mm x 241 mm, Gewicht: 857 g

Spielman / Brand

Experimental Cell Biology of Taste and Olfaction

Buch, Englisch, 464 Seiten, Format (B × H): 163 mm x 241 mm, Gewicht: 857 g

ISBN: 978-0-8493-7645-0
Verlag: CRC Press


Experimental Cell Biology of Taste and Olfaction examines and adapts methods from a variety of established fields, such as neurophysiology, receptor biochemistry and cellular imaging to provide comprehensive coverage of current techniques and protocols in chemosensory cell biology. Written for both newcomers and established scientists, this volume offers numerous tips for problem solving and suggests ways to avoid the most common, and costly, mistakes made by researchers.
This book covers general aspects such as tissue collection and preparation, as well as specific, up-to-date methods used in taste and olfactory morphology, immunology, biochemistry, biophysics, electrophysiology and molecular biology. The explosion of knowledge and the increased interest in these areas make this book an important reference work for all scientists, students, and teachers in this and related fields
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Weitere Infos & Material


General Methods in Chemosensory ResearchEuthanasia, I. RamirezCollection of Taste Tissue from Fish and Amphibians, J. H. TeeterCollection of Taste Tissue from Mammals, A. I. Spielman and J. G. BrandInsects: Isolation of Olfactory Sensilla and Collection of Sensillum Lymph, G. ZiegelbergerSurgical Anatomy of Olfactory Organ and Tissue Collection Methods in Vertebrates, T. Kurahashi and D. RestrepoCollection of Human Olfactory Tissue, L. D. Lowry and E. A. PribitkinSurgical Removal of the Vomeronasal Organ and Its Verification, C. J. Wysocki and L. M. WysockiDenervation and Reinnervation of the Tongue, B. OakleyThe Lingual Slice Preparation, Isolated Taste Cells, and Dye Injections in Mudpuppy, A. Bigiani, D. A. Ewald, and S. D. RoperCell Dissociation Methods for Patch Clamp Electrophysiological Studies of Vertebrate Olfactory Receptor Neurons, A. H. Kahn and S. FiresteinTissue Culture of Rat Taste Buds, C. Ruiz, M. McPheeters, and S. C. KinnamonOlfactory Receptor Neuronal Cultures, G. V. RonnettMethods for Airborne and Aqueous Stimulus Delivery in Olfactory Research, J. S. Kauer and S. FiresteinImaging Methods in Chemosensory ResearchVideomicroscopy and Image Processing, I. J. Miller and S. L. HayesTransmission Electron Microscopy of Gustatory Epithelium, H. H. Crowley and J. C. KinnamonScanning and Transmission Electron Microscopy of Olfactory Epithelia, B. Ph. M. MencoAnalysis of Taste Bud Ultrastructure by Serial Sectioning and Three-Dimensional Reconstruction, S. M. Royer and J. C. KinnamonImmunohistochemistry, C. DellaCorteIP3 Autoradiography of Taste Receptor Cells, P. A. HwangIn Situ Hybridization, H. Schmale and M. BehrensBiochemical Methods in Chemosensory ResearchMembrane Preparation and Subcellular Fractionation, D. L. KalinoskiPreparation of Olfactory Cilia, R. R. H. AnholtThe Use of the Spiny Lobster as a Tool for the Study of Chemosensory Biochemistry, H. G. Trapido-RosenthalReceptor Binding and Solubilization, D. L. KalinoskiWestern Blotting, R. Bruch and Fe. C. AbogadieApproaches for Monitoring Rapid Kinetics of Second Messenger Signaling, E. Tareilus, I. Boekhoff, A.I. Spielman, and H. BreerSecond Messenger Assays, A. I. Spielman, H. Nagai, G. Sunavala, M. Dasso, T. Huque, and J. G. BrandElectrophysiological and Biophysical Methods in Chemosensory ResearchGeneral MethodsReconstitution of Chemoreceptor Proteins in Planar Lipid Bilayers, A. M. Feigin, M. Zviman, and J. H. TeeterData Acquisition in Electrophysiology, V. E. DionneWhole Nerve and Single Unit RecordingsTaste Nerve Recording from a Voltage-Clamped Receptive Field, J. A. DeSimone, G. Heck, and Q. YeWhole Nerve and Single Fiber Recordings in Frog, T. Miyamoto and T. SatoSingle Unit Electrophysiological Recordings from Crustacean Chemoreceptor Neurons, C. D. DerbyOlfactory and Taste Recordings in Fish, J. CaprioTaste Nerve Recordings in Rodents, M. E. FrankTrigeminal Nerve Recordings in Rodents, B. P. BryantWhole Nerve and Single Fiber Recordings in Non-Human Primates, G. Hellekant and T. W. RobertsElectrophysiology of the Vomeronasal Organ and Accessory Olfactory System, M. MeredithPatch-ClampingPatch-Clamping of Taste Cells in the Mudpuppy, T. A. Cummings and S. C. KinnamonPatch-Clamping Recording from Taste Receptor Cells of Channel Catfish and Larval Tiger Salamander, J. H. TeeterPatch-Clamping of Taste Cells in Hamster and Rat, T. A. GilbertsonPatch-Clamping of Taste Cells in Mouse, J. F. MacDonald, I. Mody, M. W. Salter, and A. I. SpielmanLoose-Patch Recording from Taste Buds in Situ, B. LindemannPatch-Clamping Lobster Olfactory Receptor Cells, B. W. Ache, D. A. Fadool, and A. ZhainazarovPatch-Clamping of Whole Olfactory Cilia, S. J. KleenePatch-Clamping Techniques for Vertebrate Olfactory Receptor Cells, G. Lowe, T. Nakamura, and G. H. GoldSingle Unit and Electroantennogram Recordings in Insect Olfactory Organs, K.-E. KaisslingPatch-Clamping of Olfactory Cells in Insects, F. ZufallImaging of Intracellular Calcium in Chemosensory Receptor Cells, D. Restrepo, M. Zviman, and N. E. RawsonVideorate Imaging of Potentiometric Dyes in Olfactory Research, J. S. KauerMethods to Study Flow Dynamics in Olfaction, P. W. Scherer, K. Keyhani, I. Hahn, and M. MozellApplications of Molecular Biology to Chemosensory ResearchCloning of Gustatory cDNAs and Analysis of Their Pattern of Expression, P. J. McKinnon, S. K. McLaughlin, and R. F. MargolskeeCloning of Odorant Receptors, E. R. Liman and L. B. BuckList of Critical References for Techniques Not Covered


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