Genomics Protocols

2001
ISBN: 978-0-89603-708-3
Verlag: Humana Press

Buch, Englisch, Band 175, 538 Seiten, Book, Format (B × H): 152 mm x 229 mm, Gewicht: 1780 g

Reihe: Methods in Molecular Biology

We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of “bottom-up” and “top-down” approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27–30).

Zielgruppe

Research

Autoren/Hrsg.

Starkey, Michael P.

Elaswarapu, Ramnath

Inhaltsverzeichnis

Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse
Paul A. Lyons

Genetic Analysis of Complex Traits
Stephen P. Bryant and Mathias N. Chiano

Sequence-Based Detection of Single Nucleotide Polymorphisms
Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, and Mark J. Rieder

Genomic Mismatch Scanning for the Mapping of Genetic Traits
Farideh Mirzayans and Michael A. Walter

Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization
Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, and Jan P. A. Baak

Construction of a Bacterial Artificial Chromosome Library
Sangdun Choi and Ung-Jin Kim

Contiguation of Bacterial Clones
Sean J. Humphray, Susan J. Knaggs, and Ioannis Ragoussis

Mapping of Genomic Clones by Fluorescence In Situ Hybridization
Margaret A. Leversha

Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map
Panagiotis Deloukas

Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method
Yutaka Suzuki and Sumio Sugano

Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map
Panagiotis Deloukas

Preparation and Screening of High-Density cDNA Arrays with Genomic Clones
Günther Zehetner, Maria Pack, and Katja Schäfer

Direct Selection of cDNAs by Genomic Clones
Daniela Toniolo

Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System
Martin C. Wapenaar and Johan T. Den Dunnen

Sequencing Bacterial Artificial Chromosomes
David E. Harris and Lee Murphy

Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics
Yvonne J. K. Edwards and Simon M. Brocklehurst

Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning
GregElgar

Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization
Oliver Dorian von Stein

Isolation of Differentially Expressed Genes by Representational Difference Analysis
Christine Wallrapp and Thomas M. Gress

Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display
Michael P. Starkey

Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos
Nicolas Pollet and Christof Niehrs

Expression Monitoring Using cDNA Microarrays: A General Protocol
Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn, and Ronald W. Davis

Prediction of Protein Structure and Function by Using Bioinformatics
Yvonne J. K. Edwards and Amanda Cottage

Identification of Novel Genes by Gene Trap Mutagenesis
Anne K. Voss and Tim Thomas

Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice
Ahmed Mansouri

Genomic Analysis Utilizing the Yeast Two-Hybrid System
Ilya G. Serebriiskii, Garabet G. Toby, Russell L. FInley, Jr., and Erica A. Golemis

Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle
Terry J. Amiss and Richard Jude Samulski

Retroviral-Mediated Gene Transduction
Donald S. Anson

Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods
Ruth Gjerset, Ali Haghighi, Svetlana Lebedeva, and Dan Mercola

Ribozyme Gene Therapy
Leonidas A. Phylactou