Methods in Protein Biochemistry

1. Auflage 2011
ISBN: 978-3-11-025233-0
Verlag: De Gruyter

Buch, Englisch, 352 Seiten, Format (B × H): 175 mm x 246 mm, Gewicht: 900 g

This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

Zielgruppe

Graduate students, post-docs and researchers in protein biochemistry

Autoren/Hrsg.

Tschesche, Harald

Fachgebiete

• Naturwissenschaften Chemie Organische Chemie Biochemie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Chemie Analytische Chemie

Inhaltsverzeichnis

Frontmatter
Preface
Editor. List of contributing authors
Contents
Abbreviations
Acknowledgements
1 Three-phase partitioning
2 Folding and degradation functions of molecular chaperones
3 Membrane protein folding in detergents
4 Glycoprotein-folding quality control in the endoplasmic reticulum
5 Conformational dynamics in peptides and proteins studied by triplet-triplet energy transfer
6 Protein import into the intermembrane space of mitochondria
7 On-membrane identification of gel-resolved proteins by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)
8 Analysis of protein complexes using chemical cross-linking and mass spectrometry
9 Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular function
10 Wide-angle X-ray solution scattering (WAXS)
11 Where purity matters: recombinant versus synthetic peptides in beta amyloid formation
12 Chemical modifi cation of proteins in living cells
13 Proteomics of human bronchoalveolar lavage fluid: discovery of biomarkers of chronic obstructive pulmonary disease (COPD) with difference gel electrophoresis (DIGE ) and mass spectrometry (MS)
14 Proteomic analysis of Duchenne muscular dystrophy (DMD)
15 Target-oriented peptide arrays in a palliative approach to cystic fibrosis (CF)
16 Probing protein dynamics in vivo using backbone cyclization: bacterial acyl carrier protein as a case study
17 The protein epitope mimetic approach to protein-protein interaction inhibitors
18 The structural biology of a1-antitrypsin deficiency and the serpinopathies
Index

Über Autor(innen)

Tschesche, Harald
Harald Tschesche, University of Bielefeld, Germany.