Verma / Sumer Cell Reprogramming

Methods and Protocols
1. Auflage 2015
ISBN: 978-1-4939-2848-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1330, 271 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians and animal care technicians involved with reprogramming, nuclear transfer and transgenesis.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Verma, Paul J.

Sumer, Huseyin

Inhaltsverzeichnis

Part I Background1. Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative MedicineWendy DeanPart II De Novo Reprogramming2. Synthetic mRNA Reprogramming of Human Fibroblast CellsJun Liu and Paul J. Verma 3. MicroRNA Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem CellsShelley E. Swing and Bhanu Prakash V.L. Telugu4. Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid VectorsDmitry A. Ovchinnikov, Jane Sun, and Ernst J. Wolvetang5. Reprogramming of Human Fibroblasts with Non-Integrating RNA Virus on Feeder-Free or Xeno-Free ConditionsPauline T. Lieu Part III Livestock, Domestic and Endangered Species 6. Inducing Pluripotency in CattleLuis F. Malaver-Ortega, Amir Taheri-Ghahfarokhi, and Huseyin Sumer7. Generation of Induced Pluripotent Stem Cells (iPSCs) From Adult Canine Fibroblasts Sehwon Koh and Jorge A. Piedrahita8. Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a PiggyBac Transposon Delivery System and Temporal Control of Transgene ExpressionKristina Nagy and Andras Nagy9. Generation of Induced Pluripotent Stem Cells from Mammalian Endangered SpeciesInbar Friedrich Ben-Nun, Susanne C. Montague, Marlys L. Houck, Oliver Ryder, and Jeanne F. LoringPart IV Germ-Line Transmission of Pluripotent Stem Cells10. Generation of Efficient Germ-Line Chimeras Using Embryonic Stem CellsWilliam A. Ritchie11. Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation Lan Kang and Shaorong Gao12. Cloning Endangered Felids by Interspecies Somatic Cell Nuclear TransferMartha C. Gómez and C. Earle Pope 13. Generation of Chimeras from Porcine Induced Pluripotent Stem CellsFranklin D. West, Steve L. Terlouw, John R. Dobrinsky, Yangqing Lu, Erin T. Jordan, and Steven L. Stice14. A Novel Method on Somatic Cell Nuclear Transfer with the Minimum EquipmentHosseini SM, Moulavi F, and Nasr-Esfahani MH 15. Neonatal Care and Management of Foals Derived by Somatic Cell Nuclear Transfer Aime K. Johnson and Katrin HinrichsPart V Influencing Reprogramming and Genome Editing16. Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts Christian M. Nefzger, Sara Alaei, and Jose M. Polo17. Analysis of Mitochondrial DNA in Induced Pluripotent and Embryonic Stem Cells William Lee, Richard D. W. Kelly, Ka Yu Yeung, Gael Cagnone, Matthew McKenzie, Justin C. St. John18. Genome Modification of Pluripotent Cells By Using Transcription Activator-Like Effector Nucleases (TALENs) Amir Taheri-Ghahfarokhi, Luis F. Malaver-Ortega, and Huseyin Sumer

Über Autor(innen)

Professor Paul J. Verma PhD Principal Scientist- Reproductive Biology South Australian Research Institute (SARDI) Adjunct Professor & Head Cell Reprogramming & Stem Cells Monash Institute of Medical Research 27-31 Wright Street Clayton, VIC 3168 AUSTRALIA Voice: + 61 3 9902 4771 Fax: + 61 3 9594 7416 Cell: [phone] Email: [e-mail] Email: [e-mail] Huseyin Sumer Monash Institute of Medical Research Faculty of Medicine, Nursing and Health Sciences MIMR Centre for Reproduction and Development Phone: [phone] email: [e-mail]