Buch, Englisch, Band 2272, 320 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 626 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2272, 320 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 626 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-07-161296-5
Verlag: Springer US
Cutting-edge and thorough, TET Proteins and DNA Demethylation: Methods and Protocols is a valuable resource that aims to help research scientists at all levels working in the fields of DNA demethylation dynamics.
Chapters 3, 7 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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Weitere Infos & Material
Reduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine.- Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine.- Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome Bisulfite Sequencing (WGBS).- ELISA-Based Quantitation of Global 5hmC Levels.- Avidin-Biotin ELISA-Based Detection of 5hmC.- Quantification of DNA Methylation and Its Oxidized Derivatives using LC-MS.- Distinguishing Active Versus Passive DNA Demethylation using Illumina MethylationEPIC BeadChip Microarrays.- Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions.- TAB-seq and ACE-seq Data Processing for Genome-Wide DNA Hydroxymethylation Profiling.- Genomic Targeting of TET Activity for Targeted Demethylation using CRISPR/Cas9.- High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation.- Identifying Protein–(hydroxy)methylated DNA Interactions using Quantitative Interaction Proteomics.- Purification of TET Proteins.- Uncovering Sequence Specific Transcription Factors Interacting with TET2.- ChIP-Sequencing of TET Proteins.- Harnessing Alternative Substrates to Probe TET Family Enzymes.- Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts.