Capillary Electrophoresis of Proteins

1. Auflage 1998
ISBN: 978-0-8247-0205-2
Verlag: Taylor & Francis Ltd (Sales)

Buch, Englisch, 300 Seiten, Format (B × H): 160 mm x 234 mm, Gewicht: 535 g

Reihe: Chromatographic Science Series

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Autoren/Hrsg.

Wehr, Tim

Rodriguez-Diaz, Roberto

Zhu, Mingde

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Chemie Organische Chemie Polymerchemie
• Naturwissenschaften Chemie Analytische Chemie

Inhaltsverzeichnis

Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.

Über Autor(innen)

Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu