Regulated Cell Death Part A

Apoptotic Mechanisms
Erscheinungsjahr 2014
ISBN: 978-0-12-417158-9
Verlag: William Andrew Publishing

Buch, Englisch, 504 Seiten, Format (B × H): 152 mm x 229 mm, Gewicht: 1020 g

Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches.

Autoren/Hrsg.

Yuan, Junying

Wells, Jim

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie

Inhaltsverzeichnis

1. Examining the Molecular Mechanism of Bcl-2 Family Proteins at Membranes by Fluorescence Spectroscopy.
2. Photoreactive Stapled Peptides to Identify and Characterize BCL-2 Family Interaction Sites by Mass Spectrometry
3. The Structural Biology of BH3-only proteins
4. How to Analyze Mitochondrial Morphology in Healthy Cells and Apoptotic Cells in Caenorhabditis elegans
5. Apoptosis Initiation Through the Cell-Extrinsic Pathway
6. Using RNAi Screening Technologies to Interrogate the Extrinsic Apoptosis Pathway
7. Caspase Enzymology and Activation Mechanisms
8. Turning ON Caspases with Genetics and Small Molecules
9. A Multi-Pronged Approach for Compiling a Global Map of Allosteric Regulation in the Apoptotic Caspases
10. Measuring Caspase Activity in vivo
11. Single-Molecule Sensing of Caspase Activation in Live Cells via Plasmon Coupling Nanotechnology
12. In vivo Monitoring of Caspase Activation Using a Fluorescence Resonance Energy Transfer-Based Tfluorescent Probe
13. Global Analysis of Cellular Proteolysis by Selective Enzymatic Labeling of Protein N-termini
14. Complementary Methods for the Identification of Substrates of Proteolysis
15. Phospholipid Scrambling on the Plasma Membrane
16. Studying Apoptosis in the Zebrafish

Über Autor(innen)

Wells, Jim
James A. Wells, PhD, focuses on development of enabling technologies for engineering proteins and for identifying small molecules to aid in drug discovery for challenging targets such as allosteric regulation and protein-protein interactions. He is interested in the discovery and design of small molecules and enzymes that trigger or modulate cellular processes in inflammation and cancer. Using small molecules and engineered proteins, the Wells lab is studying how activation of particular signaling nodes involving protease, kinases, or ubiquitin ligases drives cell biology. The lab has focused much on a set of proteases, known as caspases, responsible for fate determining cellular decisions involved in apoptosis and innate inflammation among others. These enzymes act as cellular remodelers and help us understand the essential protein struts that support life. These targets also provide leads for developing new cancer therapeutics and biomarkers for cancer treatment.

Wells is a professor and chair of the Department of Pharmaceutical Chemistry in the UCSF School of Pharmacy. He holds a combined appointment as professor in the Department of Cellular & Molecular Pharmacology in the School of Medicine. He joined UCSF in 2005 as holder of the Harry Wm. and Diana V. Hind Distinguished Professorship in Pharmaceutical Sciences. Wells also founded and directs the Small Molecule Discovery Center (SMDC) located at UCSF's Mission Bay campus. He earned a PhD degree in biochemistry from Washington State University with Professor Ralph Yount in 1979 and completed postdoctoral work at Stanford University School of Medicine with Professor George Stark in 1982. Before joining UCSF, Wells was a founding scientist in Genentech's Protein Engineering Department and in 1998 co-founded Sunesis Pharmaceuticals.

Wells is a recipient of the Hans Neurath Award by the Protein Society, the Pfizer Award and Smissman Award given by the American Chemical Society, the Perlman Lecture Award given by the ACS Biotechnology Division, the du Vigneaud Award given by the American Peptide Society, the Merck Award from the ASBMB and in 1999 a member of the National Academy of Sciences.