Targeting Protein-Protein Interactions for Drug Discovery

1. Auflage 2025
ISBN: 978-3-527-35360-6
Verlag: Wiley-VCH GmbH

Buch, Englisch, 416 Seiten, Format (B × H): 170 mm x 244 mm

Provides comprehensive up-to-date information on current topics in protein-protein interaction drug discovery.

Autoren/Hrsg.

Zhang, Jian

Fachgebiete

• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Chemie Organische Chemie Biochemie

Inhaltsverzeichnis

Chapter 1: Exploring Protein-Protein Interactions: Concepts, Methods, and Implications
1.1 General concepts of protein-protein interactions
1.2 Functional significance of protein-protein interactions
1.3 Methods for analyzing protein-protein interactions
1.4 Implications of the basic research on protein-protein interactions
1.5 Conclusions and perspectives
 
Chapter 2: Overview of drug discovery targeting PPI systems
2.1 Introduction
2.2 Fundamentals of Protein-Protein Interactions
2.3 Challenges in Targeting PPI Systems
2.4 Approaches in Drug Discovery Targeting PPI Systems
2.5 Case Studies and Success Stories
2.6 Conclusion
 
Chapter 3: Fluorescence Resonance Energy Transfer Technology and its Applications
3.1 Introduction
3.2 Mechanism of FRET
3.3 Applications of FRET
3.4 Advantages and Limitations
3.5 Recent Advances
3.6 Conclusion
 
Chapter 4: Dissect Protein Interactions Using Mass Spectrometry
4.1 Introduction
4.2 Affinity purification coupled with mass spectrometry (AP-MS)
4.3 Proximity labeling
4.4 Cross-linking mass spectrometry (XL-MS)
4.5 Co-fractionation coupled with mass spectrometry (CF-MS)
4.6 Thermal proximity coaggregation (TPCA)
4.7 Limited proteolysis?mass spectrometry (LiP?MS)
4.8 Conclusion and outlook
 
Chapter 5: Detection of protein-protein interactions in situ via proximity ligation assay
5.1 Introduction
5.2 Implementations of proximity ligation assay
5.3 Applications of PLA for detecting protein-protein interactions
5.4 Conclusions and outlooks
 
Chapter 6: The Application of Surface Plasmon Resonance in the Characterization of Protein-Protein Interactions
6.1 Introduction
6.2. Applications of SPR assay in PPI Characterization
6.3. Advantages and Limitations of SPR Application for PPIs
6.4. Future Directions
 
Chapter 7: Computational methods for protein-protein interactions
7.1. Introduction
7.2. Protein-protein docking
7.3. End-to-end structure prediction
7.4. CAPRI experiments
7.5. Challenges and Future directions
 
Chapter 8: Foldamers as Inhibitors of Aberrant Protein-Protein Interactions
8.1 Introduction
8.2 The Evolution of Hamilton?s Oligopyridylamides
8.3 Limitations of a Tedious Synthetic Route
8.4 OPs as Antagonists of Neurodegeneration
8.5 OPs Inhibit HIV Infection
8.6 OPs Targeting Type II Diabetes
8.7 OPs Targeting and Reactivating Mutant Protein in Cancer
8.8 Novel Synthesis of OPs and Alzheimer?s Disease
8.9 2D-FAST
8.10 OQ Foldamers - Structure and Discovery
8.11 Synthesis of OQ Foldamers
8.12 OQs as Modulators of Type II Diabetes-Related aPPIs
8.13 Mechanistic Insights to OQ Manipulation of aPPIs
8.14 Chemical Diversity and Structure Modulate Efficacy of Oqs
8.15 Modulation of Alzheimer?s Disease-Related Aß
8.16 OQs for the Modulation of Synucleinopathies
8.17 Epilogue
 
Chapter 9: Application of Sulfonyl-¿-AApeptides for PPI Drug Discovery
9.1 Introduction to the Structure of Sulfonyl-¿-AA-peptides
9.2 The Applications of Sulfonyl-¿-AA-peptides
9.3 Future Directions/Conclusions
 
Chapter 10: Introduction of the Application of Stapled Peptides in Protein-Protein Interactions Drug Discovery and their Successful Examples
10.1. Introduction
10.2. Stapled Peptides: Structure Features and Benefits
10.3. Successful Applications of Stapled Peptides in Drug Discovery
10.4. Challenges and Limitations
10.5. Future Directions
 
Chapter 11: Cyclic peptides for PPI drug discovery
11.1. a-helix cyclic peptides (stapled peptides)
11.2. ß-hairpin cyclic peptides
11.3. Macrocyclic peptides
11.4. Summary and outlook
 
Chapter 12: Small molecule inhibitors targeting protein-protein interactions in the BCL protein
12.1 Introduction
12.2 Inhibitors of BCL-2 family antiapoptotic proteins
12.3 Inhibitors of ß-catenin/BCL9
12.4 Targeting BCL-6 small molecule inhibitors
12.5. BCL-3 inhibitors
12.6. BCL-10 inhibitors
12.7. Summary
 
Chapter 13: Molecular Glues as Activators for PPI
13.1 Introduction
13.2 Molecular Glues as orthosteric PPI stabilizers/activators
13.3 Methods for Molecular Glue Discovery
13.4 Conclusions and Outlook
 
Chapter 14: Targeting APC?Asef Protein-Protein Interaction for Drug Discovery in Colorectal Cancer Therapy
14.1. Introduction
14.2. Structural insights into APC?Asef interaction
14.3. Current APC?Asef inhibitor
14.4. A More Sensitive FP Method for Identifying Highly Active APC?Asef Inhibitors
14.5. Conclusions and Outlook
 
Chapter 15: Computational methods applied to drug discovery of protein-protein interaction systems
15.1 Introduction
15.2 Computational methods for PPI prediction
15.3 Conclusions and outlook
 

Über Autor(innen)

Jian Zhang received a BM degree in Pharmacology in 2002 from Peking University and a PhD in 2007 from Shanghai Institute of Materia Medica, Chinese Academy of Sciences. Then he moved to the University of Michigan to carry out postdoctoral research in Prof. Shaomeng Wang's group. In 2009, he joined Shanghai Jiao Tong University, School of Medicine, where he is a distinguished professor and doctoral supervisor. Currently, he is also the director of the Medicinal Bioinformatics Center. His fields of research include first-in-class drug design and chemical biology mainly pertaining to protein-protein interactions and allostery and their application in drug discovery.