Alexander / Lundgren A Laboratory Manual of Analytical Methods of Protein Chemistry

1. Auflage 2014
ISBN: 978-1-4831-5245-5
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 244 Seiten, Web PDF

A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 5 presents the laboratory techniques for protein and polypeptide study. This book discusses the staining procedure for histones, which has a high degree of selectivity for basic proteins and the unique ability to visualize qualitative differences in terms of color changes. Organized into four chapters, this volume begins with an overview of the formalin-mediated ammoniacal-silver staining procedure as a selective stain for basic proteins and its application per cell and per extract. This text then examines the optical rotatory dispersion (ORD), which has advanced into a powerful tool for describing the conformations and conformational changes of biopolymers. Other chapters consider the application of ultrasensitive calorimetry to thermodynamic problems. This book discusses as well the principle of the technique, its instrumentation, and experimental procedures. The final chapter deals with the hydrodynamic densities and preferential hydration values for protein precipitates in concentrated salt solutions. This book is a valuable resource for chemists and biochemists.

Autoren/Hrsg.

Alexander, P.

Lundgren, H. P.

Inhaltsverzeichnis

1;Front Cover;1
2;A Laboratory Manual of Analytical Methods of Protein Chemistry;4
3;Copyright Page;5
4;Table of Contents;6
5;PREFACE TO VOLUME 5;7
6;PREFACE TO VOLUME 4;8
7;PREFACE TO VOLUMES 1, 2 and 3;9
8;LIST OF PREVIOUS CONTENTS;10
9;CHAPTER 1. SELECTIVE STAINING OF HISTONES WITH AMMONIACAL-SILVER;12
9.1;I. INTRODUCTION;14
9.2;II. A-S STAINING;16
9.3;III. COMPARISON OF FG AND A-S STAINING;26
9.4;IV. APPLICATIONS;29
9.5;V. SUMMARY;33
9.6;REFERENCES;34
10;CHAPTER 2. OPTICAL ROTATORY DISPERSION AND CIRCULAR DICHROISM;36
10.1;1. INTRODUCTION;38
10.2;2. PRINCIPLES AND DEFINITIONS;39
10.3;3. INSTRUMENTATION;47
10.4;4. METHODS;60
10.5;5. EXPERIMENTAL OBSERVATIONS;63
10.6;6. PHENOMENOLOGICAL EQUATIONS;76
10.7;7. ESTIMATION OF THE SECONDARY STRUCTURES IN PROTEINS;81
10.8;8. CONFORMATIONAL TRANSITIONS;91
10.9;9. CONCLUSIONS;97
10.10;ACKNOWLEDGEMENTS;98
10.11;APPENDIX;99
10.12;REFERENCES;102
11;CHAPTER 3. ULTRASENSITIVE REACTION CALORIMETRY;106
11.1;INTRODUCTION;108
11.2;A. INSTRUMENTATION;106
11.3;B. EXPERIMENTAL PROCEDURES;130
11.4;C. ANALYTICAL APPLICATIONS OF ULTRASENSITIVE REACTION CALORIMETRY: CONCRETE EXAMPLES;137
11.5;D. THERMODYNAMIC MEASUREMENTS BY ULTRASENSITIVE CALORIMETRY;152
11.6;CONCLUSIONS;173
11.7;ACKNOWLEDGEMENTS;174
11.8;REFERENCES;175
12;CHAPTER 4. PROTEINS AT SEDIMENTATION EQUILIBRIUM IN DENSITY GRADIENTS;178
12.1;I. INTRODUCTION;180
12.2;II. THEORY;184
12.3;III. EXPERIMENTAL;205
12.4;IV. APPLICATIONS;234
12.5;ACKNOWLEDGEMENTS;249
12.6;APPENDIX A:;250
12.7;APPENDIX B:;251
12.8;REFERENCES;252
13;AUTHOR INDEX;256
14;SUBJECT INDEX;260