Redox Chemistry and Biology of Thiols

Erscheinungsjahr 2022
ISBN: 978-0-323-90219-9
Verlag: Elsevier Science

Buch, Englisch, 746 Seiten, Format (B × H): 190 mm x 236 mm, Gewicht: 450 g

Redox Chemistry and Biology of Thiols offers an applied, comprehensive overview of redox chemistry and biology of thiol-dependent processes. Running from basic biology and chemistry of redox phenomena to research methods and highlighting recently identified roles of thiols across cellular and bodily systems, this book draws upon a range of disciplines to illuminate new research directions, new applications of thiol studies, and clinical translation. Sections cover thiol oxidizing species, thiol reactivity and modifications, thioredoxin, glutaredoxin, glutathione, peroxidases, thiol repair enzymes, thiol oxidative signaling, disulfide bond formation, thiol-based redox biosensors, cysteine and hydrogen sulfide metabolism, iron-sulfur cluster biogenesis, thiols in chloroplasts, blood thiols, sugar and polyamine thiols in pathogenic organisms, redox medicine (therapeutic applications of thiols and drug development), as well as methods and bioinformatics tools.

Zielgruppe

Life science researchers in biochemistry, molecular biology, and cell biology; clinical chemists and pharmaceutical scientists; and medical and analytical chemists Clinicians and students

Autoren/Hrsg.

Alvarez, Beatriz

Comini, Marcelo

Salinas, Gustavo

Trujillo, Madia

Fachgebiete

• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1. Basic concepts of thiol chemistry and biology 2. Chemical basis of cysteine reactivity: Acidity and nucleophilicity 3. Computational functional analysis of cysteine residues in proteins 4. Global approaches for protein thiol redox state detection and quantification 5. Thiol oxidation by biologically-relevant reactive species 6. Thiyl radicals: formation, properties and detection 7. Detection of the oxidation products of thiols: disulfides, sulfenic, sulfinic and sulfonic acids 8. Biochemistry and detection of S-nitrosothiols 9. Thiol modification and signaling by biological electrophiles 10. Thioredoxin and glutathione reductases 11. Functional plasticity in the thioredoxin family: FeS-thio- and glutaredoxins 12. Glutathione and glutathione-dependent enzymes 13. Thiol and selenol-based peroxidases: Structure and catalytic properties 14. Thiol peroxidase-based redox relays 15. Compartmentalized disulphide bond formation pathways 16. Disulfide bond formation in Escherichia coli 17. Thiol-based redox probes 18. Selenocysteine-containing proteins 19. Overview of cysteine metabolism 20. Hydrogen sulfide and persulfides 21. The role of thiols in iron-sulfur cluster biogenesis 22. Thiol-based redox control in chloroplasts 23. Sugar-based cysteine thiols recruited for oxidative stress defence and redox regulation 24. Polyamine-based thiols in pathogens 25. Thiols in blood 26. A thiol chemistry perspective on redox medicine 27. Therapeutic applications of low-molecular-weight thiols and selenocompounds 28. Thiol targets in drug development to combat bacterial infections

Über Autor(innen)

Alvarez, Beatriz
Dr. Beatriz Alvarez is a Professor at the Laboratory of Enzymology, School of Sciences, Universidad de la República, Montevideo, Uruguay. Her research interests span the areas of redox biochemistry, kinetics and enzymology, especially in relation to biological thiols and hydrogen sulfide.

Trujillo, Madia
Madia Trujillo, MD, PhD, is an Associate Professor of Biochemistry at the School of Medicine and an Investigator at the Centro de Investigaciones Biomédicas, Universidad de la República, Uruguay. Her research focuses on the characterization of the reactions of biologically relevant oxidants as well as in the mechanisms of their sensing and detoxification, particularly in humans and human pathogens.

Salinas, Gustavo
Dr. Gustavo Salinas is a Principal Investigator at the Worm Biology Laboratory, Institut Pasteur de Montevideo, Uruguay, as well as a Professor at the Department of Biosciences, School of Chemistry, Universidad de la República, Uruguay. His research contributes to the understanding of linked thioredoxin-glutathione systems in parasites, glutathione-independent deglutathionylation, selenocysteine horizontal gene transfer and the use of selenocysteine in fungi. He is also interested in anaerobic mitochondria and recently discovered key steps in the biosynthesis of rhodoquinone, an electron transporter used in anaerobic bioenergetics.

Comini, Marcelo
Dr. Marcelo Comini is a Principal Investigator at the Redox Biology of Trypanosomes Laboratory, Institut Pasteur de Montevideo, Uruguay. He is a Biochemist (Universidad Nacional del Litoral, Argentina) and Dr. rer. nat. from the Technische Universität Brausnchweig (Germany). The research interest of his lab encompasses understanding the role of different components of the thiol-redox system of trypanosomatids, the identification of inhibitors thereof, and the development of fluorescent-protein redox biosensors.