Plant Protoplasts and Genetic Engineering III

1993
ISBN: 978-3-540-56313-6
Verlag: Springer

Buch, Englisch, Band 22, 332 Seiten, Book, Format (B × H): 170 mm x 242 mm, Gewicht: 810 g

Reihe: Biotechnology in Agriculture and Forestry

Zielgruppe

Research

Autoren/Hrsg.

Bajaj, Y. P. S.

Inhaltsverzeichnis

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Agrostis alba (Redtop).- 1 Introduction.- 2 Induction of Embryogenic Callus and Establishment of Embryogenic Suspension Culture.- 3 Isolation of Protoplasts.- 4 Culture of Protoplasts.- 5 Regeneration of Plants.- 6 Conclusions.- 7 Protocol.- References.- I.2 Regeneration of Plants from Protoplasts of Atropa belladonna L. (Deadly Nightshade).- 1 Introduction.- 2 Isolation and Culture of Protoplasts.- 3 Protoplast Fusion and Somatic Hybridization.- 4 Cryopreservation of Protoplasts.- 5 Summary.- 6 Protocol.- References.- I.3 Regeneration of Plants from Protoplasts of Brassica alboglabra Bailey (Chinese Kale).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration and Acclimatization of Plants.- 5 Characterization of Protoplast-Derived Plants.- 6 Gene Transfer into Protoplasts Using Electroporation.- 7 Conclusions and Prospects.- 8 Protocol.- References.- I.4 Regeneration of Plants from Protoplasts of Cucumis sativus L. (Cucumber).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Fusion of Protoplasts.- 6 Conclusions and Prospects.- 7 Protocol for Isolation, Culture, and Regeneration of Cucumber Protoplasts.- References.- I.5 Regeneration of Plants from Protoplasts of Lolium (Ryegrasses) and Festuca (Fescues).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Culture Media and Conditions.- 5 Regeneration from Protoplast Colonies.- 6 Somaclonal Variation in Regenerated Plants.- 7 Summary and Conclusions.- 8 Protocol.- References.- I.6 Regeneration of Plants from Protoplasts of Lotus spp. (Birdsfoot trefoil).- 1 Introduction.- 2 Protoplast Isolation.- 3 Protoplast Culture and Plant Regeneration.- 4 Conclusion and Prospects.- 5 Protocol.- References.- I.7 Regeneration of Plants from Protoplasts of Lycium barbarum L. (Wolfberry).- 1 Introduction.- 2 Plant Material and Callus Culture.- 3 Mesophyll and Callus Protoplast Isolation.- 4 Mesophyll Protoplast Culture and Plant Regeneration.- 5 Culture of Callus-Derived Protoplasts.- 6 Summary and Conclusions.- References.- I.8 Regeneration of Plants from Protoplasts of Pelargonium spp. (Geranium).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Plastid Inheritance.- 6 Conclusion.- 7 Protocols.- References.- I.9 Regeneration of Plants from Protoplasts of Pea (Pisum sativum L.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Protoplast Culture.- 4 Regeneration of Plants.- 5 Protocol.- References.- I.10 Regeneration of Plants from Protoplasts of Pyrus spp. (Pear).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Protoplast Fusion and Somatic Hybridization Studies with Pyrus Protoplasts.- 6 Miscellaneous Studies with Pyrus Protoplasts.- 7 Summary and Conclusion.- 8 General Protocols.- References.- Section II Genetic Transformation.- II.1 Transformation in Arabidopsis thaliana.- 1 Introduction.- 2 Plant Culture and Protoplast Isolation.- 3 Direct Gene Transfer to Protoplasts.- 4 Selection of Transformed Colonies.- 5 Plant Regeneration.- 6 Concluding Remarks.- References.- II.2 Transformation in Atropa belladonna.- 1 Introduction.- 2 Preparation of Agrobacterium.- 3 Transformation.- 4 Elimination of Bacteria and Selection of Transformed Cells.- 5 Regeneration of Plants.- 6 Transgenic A. belladonna.- 7 Conclusion.- 8 Protocols for Transformation.- References.- II.3 Transformation in Catharanthus Species (Madagascar Periwinkle).- 1 Introduction.- 2 Importance of Catharanthus.- 3 Genetic Transformation of Catharanthus Species.- 4 Potential of Hairy Root Cultures.- 5 Transformation of C. roseus and Plant Pathology.- 6 Genetic Engineering with Genes Involved in Tropane Alkaloids Synthesis.- 7 Conclusion.- References.- II.4 Transformation in Datura Species.- 1 Introduction.- 2 Tropane Alkaloid Production.- 3 Transformation.- 4 Summary and Conclusion.- References.- II.5 Transformation in Daucus carota L. (Carrot).- 1 Introduction.- 2 Stable Genetic Transformation of Carrot.- 3 Applications of Electroporation of Carrot Protoplasts.- 4 Isolation and Culture of Carrot Protoplasts.- 5 Electroporation Parameters.- 6 Protocol for the Establishment of an Electroporation System.- 7 Conclusion.- References.- II.6 Transformation in Digitalis purpurea L. (Foxglove).- 1 Introduction.- 2 Transformation.- 3 Expression of Chimeric Genes in Transgenic Digitalis Roots.- 4 Production of Cardioactive Glycosides.- 5 Summary and Conclusions.- 6 Protocols.- References.- II.7 Transformation in Duboisia spp..- 1 Introduction.- 2 Genetic Transformation in Duboisia by the Ri T-DNA of Agrobacterium rhizogenes.- 3 Somatic Hybrids Between Duboisia and Nicotiana by Protoplast Fusion.- 4 Summary and Conclusions.- References.- II.8 Transformation in Eustoma grandiflorum (Lisianthus).- 1 Introduction.- 2 Breeding and In Vitro Propagation.- 3 Agrobacterium-Mediated Transformation.- 4 Analysis of Early Transformation Events.- 5 Production of Kanamycin-Resistant Shoots.- 6 Southern Hybridization Analysis.- 7 Future Directions: Manipulation of Color in Lisianthus.- References.- II.9 Transformation in Helianthus annuus L. (Sunflower).- 1 Introduction.- 2 Methodology and Experimental Results.- 3 Summary and Conclusions.- References.- II.10 Transformation in Hordeum vulgare (Barley).- 1 Introduction.- 2 Culture of Barley Coleoptile Tissues.- 3 Transient Expression of Foreign Genes Introduced into Barley Coleoptile Cells by Microinjection.- 4 Assessment of Transformation Strategy with Respect to Disease Resistance.- 5 Promoter Sequences of Barley Responsible for Infection with Powdery Mildew Pathogen.- 6 Conclusion and Prospects.- References.- II.11 Transformation in Kalanchoe.- 1 Introduction.- 2 Transient Gene Expression.- 3 Stable Transformation.- 4 Transformation Protocol.- 5 Perspectives.- References.- II.12 Transformation in Linum usitatissimum L. (Flax).- 1 Introduction.- 2 Agrobacterium-Mediated Flax Transformation.- 3 Genetic Engineering for Flax Crop Improvement.- 4 Summary and Conclusions.- References.- II.13 Transformation in Lobelia inflata.- 1 Introduction.- 2 Establishment of Hairy Root Culture and Production of Secondary Metabolites.- 3 Results and Discussion.- 4 Summary and Conclusions.- References.- II.14 Transformation in Lycopersicon esculentum L. (Tomato).- 1 Introduction.- 2 Parameters Important for the Preparation and Culture of Tomato Protoplasts.- 3 Direct Gene Transfer into Mesophyll Protoplasts of L. esculentum and L. peruvianum by Electroporation.- 4 Conclusion.- References.- II.15 Transformation in Mentha Species (Mint).- 1 Introduction.- 2 Procedures for the Genetic Manipulation of Mentha.- 3 Phenotypes of Mentha Galls Transformed by Different Strains of A. tumefaciens.- 4 Analysis of Transformed Shoot Cultures.- 5 Summary and Conclusions.- References.- II.16 Transformation in Nicotiana edwardsonii.- 1 Introduction.- 2 Leaf Disc Transformation and Regeneration.- 3 Transient Expression of Introduced Genes in Nicotiana edwardsonii.- 4 Conclusion.- References.- II.17 Transformation in Salvia chinensis.- 1 Introduction.- 2 Plant Regeneration System.- 3 Transgenic Plants and Shoots Produced by Agrobacterium-Mediated Transformation.- 4 Protocol.- 5 Conclusions.- References.- II.18 Transformation in Scopolia.- 1 Introduction.- 2 Cell Culture of Scopolia.- 3 Ri Plasmid Transformation and Hairy Root Culture.- 4 Summary and Conclusions.- References.- II.19 Transformation in Solanum melongena L. (Eggplant).- 1 Introduction.- 2 General Transformation Strategies.- 3 Eggplant Transformation by Cocultivation.- 4 Transgenic Somatic Embryos.- 5 Conclusions.- References.