PDZ Mediated Interactions

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-0716-1165-4
Verlag: Springer US

Buch, Englisch, Band 2256, 292 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 772 g

Reihe: Methods in Molecular Biology

This volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field. 

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Borg, Jean-Paul

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Identification of PDZ Interactions by Yeast Two-Hybrid.- Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis.- Identification of PDZ Interactions by Proteomic Peptide Phage Display.- A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay.- Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore.- PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations.- Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module.- A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics.- Unveiling the Folding Mechanism of PDZ Domains.- Development of Peptide-Based PDZ Domain Inhibitors.- Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs.- Chemical Synthesis of PDZ Domains.- Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains.- Computational Design of PDZ:peptide Binding.- Mechanoregulation of PDZ Proteins, An Emerging Function.- Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains.