Cal / Obaya Proteases and Cancer

Methods and Protocols
1. Auflage 2018
ISBN: 978-1-4939-7595-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1731, 352 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography,  angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Proteases and Cancer: Methods and Protocols is a valuable resource for clinical researchers across numerous fields such as molecular and cellular biology, oncology, biochemistry, and chemistry. This book is also useful for PhD students in the various disciples of health sciences.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Cal, Santiago

Obaya, Alvaro J.

Inhaltsverzeichnis

dissecting Degradomes: Analysis of Protease-Coding Genes.- Identification of Protease Cleavage Sites and Substrates in Cancer by Carboxy-TAILS (C-TAILS).- Identification of Proteolytic Cleavage Sites of EphA2 by Membrane Type-1 Matrix Metalloproteinase on the Surface of Cancer Cells.- Biotin Chasing Assay to Evaluate uPAR Stability and Cleavage on the Surface of Cells.- Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease.- A Simple Cell-Based Assay for the Detection of Surface Protein Shedding by Rhomboid Proteases.- Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm.- Autophagy and Proteases: Basic Study of the Autophagic Flux by Western Blot.- Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture.- Analysis of Enzymatic Activity of Matrix Metalloproteinase (MMP) by Collagen Zymography in Melanoma.- Measurement of Protease Activities using Fluorogenic Substrates.- Targeting theExpression of Cathepsin B using CRISPR/Cas9 System in Mammalian Cancer Cells.- Assessing the Influence of a Protease in Cell Migration using the Barrier-Migration Assay.- Analysis of Invasive Activity of CAF-Spheroids into Three-Dimensional (3D) Collagen Matrices.- Beyond the Proteolytic Activity: Examining the Functional Relevance of the Ancillary Domains using Tri-Dimensional (3D) Spheroid Invasion Assay.- Analyzing the Type II Transmembrane Serine Protease Hepsin-Dependent Basement Membrane Remodeling in 3D Cell Culture.- Evaluation of Tumor Vasculature using a Syngeneic Tumor Model in Wild Type and Genetically Modified Mice.- 3D Image Analysis of the Microvasculature in Healthy and Diseased Tissues.- Human Tumor Tissue-Based 3D In Vitro Invasion Assays.- Ear Sponge Assay: A Method to Investigate Angiogenesis and Lymphangiogenesis in Mice.- Cancer Susceptibility Models in Protease-Deficient Mice.- Imaging Proteolytic Activities in Mouse Models of Cancer.- Protease Silencing to Explore the Molecular Mechanisms of Cancer and Aging.- Analysis of Somatic DNA Methylation Alterations of Genes Encoding Cell Surface Metallopeptidases in Colorectal Cancer.- Targeting Deubiquitinases in Cancer.- Generation of Highly Selective MMP Antibody Inhibitors.- Strategies to Target Matrix Metalloproteinases as Therapeutic Approach in Cancer.