E-Book, Englisch, 250 Seiten, Web PDF
Cauwenberge / Franchimont / Alexander Assay of Protein and Polypeptide Hormones
1. Auflage 2014
ISBN: 978-1-4831-4596-9
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark
International Series of Monographs in Pure and Applied Biology Modern Trends in Physiological Sciences
E-Book, Englisch, 250 Seiten, Web PDF
ISBN: 978-1-4831-4596-9
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark
International Series of Monographs in Pure and Applied Biology, Modern Trends in Physiological Sciences, Volume 33: Assay of Protein and Polypeptide Hormones focuses on the assays of protein and polypeptide hormones. The selection first discusses the general principles of protein and polypeptide hormone assays, including conditions and basic factors for bioassays and status of biological assays. The book looks at the assays of hypophyseal hormones, such as growth hormones, adrenocorticotropin (ACTH), thyrotropin, gonadotropins, prolactin, vasopressin, and oxytocin. Concerns include radioimmunoassay, standards of thyrotropin, immunoassay of hypophyseal gonadotropins, and bioassay of prolactin. The book evaluates the assays of pancreatic hormones, such as insulin and glucagon. Chemical structure and properties of insulin and radioimmunoassay of glucagon are explained. The text also focuses on the assays of erythropoietin, renin, human chorionic gonadotropin, and human placental lactogen (HPL). Concerns include augmentation of red cell volume; biological application of renin assays; and similarities and differences between hypophyseal somatotropin and HPL. The selection is a valuable source of data for readers interested in the assays of protein and polypeptide hormones.
Autoren/Hrsg.
Weitere Infos & Material
1;Front Cover;1
2;Assay of Protein and Polypeptide Hormones;4
3;Copyright Page;5
4;Table of Contents;6
5;PART I: INTRODUCTION;8
5.1;Introduction;10
6;PART II: GENERAL PRINCIPLES OF PROTEIN AND POLYPEPTIDE HORMONE ASSAY;12
6.1;CHAPTER 1. Biological Methods;14
6.1.1;I. Essential Conditions for Bioassay;14
6.1.2;II. Basic Factors in Bioassay;15
6.1.3;III. Current Status of Biological Assays;17
6.1.4;References;18
6.2;CHAPTER 2. Immunological Methods;19
6.2.1;I. Essential Conditions for Immunoassay;19
6.2.2;II. Techniques for Visualizing the Immunochemical Reaction;23
6.2.3;References;36
7;PART III: ASSAY OF HYPOPHYSEAL HORMONES;40
7.1;CHAPTER 3. Growth Hormone;42
7.1.1;I. Bioassay Techniques;42
7.1.2;II. Immunological Methods;43
7.1.3;III. Radiommunoassay;49
7.1.4;References;65
7.2;CHAPTER 4. Adrenocortícotropin (ACTH);70
7.2.1;I. Bioassay of ACTH;70
7.2.2;II. Radioimmunoassay of ACTH;73
7.2.3;References;81
7.3;CHAPTER 5. Thyrotropin;84
7.3.1;I. Introduction;84
7.3.2;II. Standards of Thyrotropin;85
7.3.3;III. Bioassays;86
7.3.4;IV. Immunoassays;90
7.3.5;V. Reliability of the Various Assay Methods for Thyrotropin;93
7.3.6;VI. Thyrotropin in Human Serum or Plasma;95
7.3.7;VII. General Conclusions;96
7.3.8;References;98
7.4;CHAPTER 6. The Gonadotropins;101
7.4.1;I. Bioassay Techniques;101
7.4.2;II. Immunoassay of Hypophyseal Gonadotropins;104
7.4.3;III. Radioimmunoassay;105
7.4.4;References;133
7.5;CHAPTER 7. Prolactin;136
7.5.1;I. Bioassay of Prolactin;136
7.5.2;II. Immunoassay of Prolactin;138
7.5.3;References;138
7.6;CHAPTER 8. Vasopressin and Oxytocin;140
7.6.1;I. Bioassays;140
7.6.2;II. Radioimmunoassays;148
7.6.3;References;151
8;PART IV: ASSAY OF PANCREATIC HORMONES;153
8.1;CHAPTER 9. Insulin;155
8.1.1;I. Chemical Structure and Properties of Insulin;155
8.1.2;II. Assay Methods;158
8.1.3;References;171
8.2;CHAPTER 10. Glucagon;175
8.2.1;I. Introduction;175
8.2.2;II. Bioassays;177
8.2.3;III. Radioimmunoassay;180
8.2.4;References;190
9;PART V: ASSAY OF RENAL HORMONES;195
9.1;CHAPTER 11. Erythropoietin;197
9.1.1;I. Augmentation of Red Cell Volume;197
9.1.2;II. Uptake of Fe59 in the rat;198
9.1.3;III. Uptake of Fe59 in the Polycythemic Mouse;200
9.1.4;IV. Standards;203
9.1.5;References;203
9.2;CHAPTER 12. Renin;205
9.2.1;I. Introduction;205
9.2.2;II. Components of the Renin—Angiotensin System;206
9.2.3;III. Assay Methods;209
9.2.4;IV. Biological Application of Renin Assays;214
9.2.5;References;215
10;PART VI: ASSAY OF HORMONES REGULATING THE METABOLISM OF PHOSPHORUS AND CALCIUM;219
10.1;CHAPTER 13. Parathormone;221
10.1.1;I. Bioassays;221
10.1.2;II. Radioimmunoassay;223
10.1.3;References;225
10.2;CHAPTER 14. Thyrocalcitonin;226
10.2.1;I. Bioassay;226
10.2.2;References;232
11;PART VII: ASSAY OF PLACENTAL HORMONES;233
11.1;CHAPTER 15. Human Chorionic Gonadotropin;235
11.1.1;I. Bioassays;235
11.1.2;II. Immunoassays;235
11.1.3;III. Results;237
11.1.4;References;238
11.2;CHAPTER 16. Human Placental Lactogen;239
11.2.1;I. Similarities and Differences Between STH and HPL;239
11.2.2;II. Radioimmunoassay;243
11.2.3;References;244
12;Index;245
13;Other Titles in the Division Modern Trends in Physiological Sciences;255
