E-Book, Englisch, Band 249, 240 Seiten, eBook
Reihe: Methods in Molecular Biology
De Ley Cytokine Protocols
Erscheinungsjahr 2008
ISBN: 978-1-59259-667-6
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, Band 249, 240 Seiten, eBook
Reihe: Methods in Molecular Biology
ISBN: 978-1-59259-667-6
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark
Zielgruppe
Research
Autoren/Hrsg.
Weitere Infos & Material
Large-Scale Generation of Plasmids that Express Type I Interferon.- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay.- Modulation of the Interferon-? Signal by Transfection of Cells with an Antisense-RNA Expressing Vector.- Competitive RT-PCR to Quantify Small Amounts of mRNA.- In SituHybridization for Cytokines in Human Tissue Biopsies.- Purification, Identification, and Synthesis of Chemokines.- Receptor Isolation and Characterization.- Crystallization of Cytokine-Receptor Complexes.- Biosensor Analysis of Receptor-Ligand Interactions.- Analysis of SH2 Ligands and Identification of Sites of Interaction.- Assays for Antiviral Activity.- Assays for Cytotoxicity.- Assays for Chemotaxis.- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor.- Development of a Mammalian Tet-On Expression Cell Line.- Immunohistochemical Detection of Cytokines in Human Tissue Sections.- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level.- Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation.- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.
