Plant Organogenesis

Methods and Protocols
2013
ISBN: 978-1-62703-220-9
Verlag: Humana Press

Buch, Englisch, Band 959, 356 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 8416 g

Reihe: Methods in Molecular Biology

Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

De Smet, Ive

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik

Inhaltsverzeichnis

1. The Tomato Leaf as a Model System for Organogenesis

            Yogev Burko and Naomi Ori

2. Usefulness of Physcomitrella patens for Studying Plant Organogenesis

            Sandrine Bonhomme, Fabien Nogué, Catherine Rameau, and Didier G. Schaefer

3. The Dicot Root as a Model System for Studying Organogenesis

            Julie Lavenus, Michael Lucas, Laurent Laplaze, and Soazig Guyomarc’h

4. Genetic Control of Root Organogenesis in Cereals

            Caroline Marcon, Anja Paschold, and Frank Hochholdinger

5. Gene Expression Analysis of Aquatic Angiosperms Podostemaceae to Gain Insight into the Evolution of Their Enigmatic Morphology

            Satoshi Koi and Natsu Katayama

6. Brown Algae as a Model for Plant Organogenesis

            Kenny A. Bogaert, Alok Arun, Susana M. Coelho, and Olivier De Clerck

7. Microscopic Analysis of Ovule Development in Arabidopsis thaliana

            Balaji Enugutti, Maxi Oelschner, and Kay Schneitz

8. Imaging of Phenotypes, Gene Expression and Protein Localization During Embryonic Root Formation in Arabidopsis

            Cristina Llavata-Peris, Annemarie Lokerse, Barbara Möller, Bert De Rybel, and Dolf Weijers

9. Inducible System for Lateral Roots in Arabidopsis thaliana and Maize

            Leen Jansen, Boris Parizot, and Tom Beeckman

10. Adventitious Root Induction in Arabidopsis thaliana as a Model for in vitro Root Organogenesis

            Inge Verstraeten, Tom Beeckman, and Danny Geelen

11. High-Throughput, Non-Invasive Imaging of Root Systems

            Anjali S. Iyer-Pascuzzi, Paul R. Zurek, and Philip N. Benfey

12. Small Molecule Screens to Study Lateral Root Development

            Dominique Audenaert, Bert De Rybel, Long Nguyen, and Tom Beeckman

13. Cell Lineage Analyses in Living Tissues

            John Runions and Smita Kurup

14. Protein Immunolocalization in Maize Tissues

            Cristian Forestan, Nicola Carraro, and Serena Varotto

15. Auxin Immunolocalization in Plant Tissues

            Cristian Forestan and Serena Varotto

16. Gene Expression Analysis of Shoot Apical Meristem Cell Types

            Ram Yishor Yadav, Stephen Snipes, Thomas Girke, and G. Venugopala Reddy

17. Kinematic Analysis of Cell Division in Leaves of Mono- and Dicotyledonous Species: A Basis for Understanding Growth and Developing Refined Molecular Sampling Strategies

            Hilde Nelissen, Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabuo Fiorani, and Gerrit T.S. Beemster

18. Regeneration in Arabidopsis Tissue Culture

            Kaoru Sugimoto and Elliot M. Meyerowitz

19. Isolation and Analysis of mRNAs from Specific Cell Types of Plants by Ribosome Immunopurification

            Angelika Mustroph, Eugenia Zanetti, Thomas Girke, and Julia Bailey-Serres

20. Analyzing Small and Long RNAs in Plant Development Using Non-Radioactive in-situ Hybridization

            Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi

21. Analyzing Protein Distribution in Plant Tissues Using “Whole-Mount” Immunolocalization

            Pilar Bustons-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi

22. Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus

            Aude Le Bail and Bénédicte Charrier

23. Building Simulation Models of Developing Plant Organs Using VirtualLeaf

            Roeland M.H. Merks and Michael Guravage