DeLucas Membrane Protein Crystallization

1. Auflage 2009
ISBN: 978-0-08-096159-0
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, Band Volume 63, 336 Seiten

Reihe: Current Topics in Membranes

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization.
This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Autoren/Hrsg.

DeLucas, Larry

Inhaltsverzeichnis

1;Front Cover;1
2;Membrane Protein Crystallization;2
3;Copyright Page;5
4;Dedication Page;7
5;Contents;8
6;Contributors;12
7;Preface;16
8;Previous Volumes in Series;18
9;Introduction;22
10;Chapter 1: Introduction to the Crystallization of Biological Macromolecules;26
10.1;I. Overview;26
10.2;II. Introduction;27
10.3;III. The Requirement for Supersaturation;27
10.4;References;43
11;Chapter 2: In Vitro Synthesis of Posttranslationally Modified Membrane Proteins;46
11.1;I. Overview;46
11.2;II. Introduction;47
11.3;III. Materials and Methods;50
11.4;IV. Results;51
11.5;V. Conclusions;67
11.6;Acknowledgments;68
11.7;References;68
12;Chapter 3: Harnessing Photosynthetic Bacteria for Membrane Protein Production;72
12.1;I. Overview;73
12.2;II. Introduction;73
12.3;III. Foreign Gene Expression in Rhodobacter sphaeroides;78
12.4;IV. Membrane Protein Preparations for Structural and Functional Studies;89
12.5;V. Practical Aspects of Heterologous Protein Expression in Rhodobacter;94
12.6;Concluding Statements;98
12.7;Acknowledgments;99
12.8;References;99
13;Chapter 4: Monoacylglycerols: The Workhorse Lipids for Crystallizing Membrane Proteins in Mesophases;104
13.1;I. Overview;104
13.2;Introduction;105
13.3;III. In Meso Crystallogenesis in Molecular Detail;107
13.4;IV. Mesophase Behavior: From Lamellar to Cubic and Sponge Phases;108
13.5;VI. Lipid Synthesis and Purification;116
13.6;VII. Conclusions;126
13.7;Acknowledgments;127
13.8;References;127
14;Chapter 5: Practical Aspects of Membrane Proteins Crystallization in Bicelles;130
14.1;I. Overview;130
14.2;II. Introduction;131
14.3;III. Bicelles;133
14.4;IV. Phase Behavior of Bicelles;134
14.5;V. Examples of Membrane Proteins Crystallized in Bicelles;135
14.6;VI. Crystal Packing;139
14.7;VII. Practical Information;139
14.8;VIII. Conclusion;143
14.9;References;144
15;Chapter 6: Membrane Protein Crystallization: Approaching the Problem and Understanding the Solutions;148
15.1;I. Overview;149
15.2;II. Introduction;149
15.3;III. The Basic Crystallization Process;149
15.4;IV. Vapor Diffusion;152
15.5;V. Microdialysis;154
15.6;VI. Microfluidics;155
15.7;VII. Counter-Diffusion;155
15.8;VIII. Novel Methods using Lipids in Crystallization;156
15.9;IX. How to obtain Crystals from a Membrane Protein of Choice;158
15.10;X. Optimization of Initial "Hits";160
15.11;XI. Seeding;161
15.12;XII. Is it Possible to Preselect Crystallization Conditions That are Inherently More Favorable for Crystallization?;165
15.13;XIII. Conclusions;166
15.14;Acknowledgments;167
15.15;References;167
16;Chapter 7: Tools to Enhance Membrane Protein Crystallization;172
16.1;I. Overview;173
16.2;II. Introduction;173
16.3;III. Protein-Protein Interactions and Crystallization;174
16.4;Self-Interaction Chromatography;177
16.5;V. Validation Studies: SIC Accurately Quantifies B;179
16.6;References;196
17;Chapter 8: Advances in Microfluidic Membrane Protein Crystallization Techniques;200
17.1;I. Overview;200
17.2;II. Membrane Protein Crystallization;201
17.3;III. Microfluidics;201
17.4;IV. Crystallization by FID in Polydimethylsiloxane (PDMS) Devices;203
17.5;V. Membrane Protein Crystallization in Gradients Established in Microchannels;205
17.6;VI. Plug-Based Membrane Protein Crystallization in Microcapillaries;206
17.7;VII. Conclusion;208
17.8;Acknowledgments;209
17.9;References;210
18;Chapter 9: Crystallization of Photosynthetic Membrane Proteins;212
18.1;I. Overview;213
18.2;II. Overview of Crystallization of Photosynthetic Membrane Proteins;213
18.3;III. Biological Parameters that Influence Crystallization;217
18.4;IV. Physical-Chemical Parameters that Influence Crystallization;223
18.5;V. Crystallization Techniques;230
18.6;VI. Determination of Phase Diagrams;237
18.7;VII. Seeding Techniques;242
18.8;References;245
19;Chapter 10: A Practical Guide to X-Ray Crystallography of beta-barrel Membrane Proteins: Expression, Purification, Detergent Selection, and Crystallization;250
19.1;I. Overview;251
19.2;II. Introduction;251
19.3;III. Expression and Purification;253
19.4;IV. Preliminary Crystallization and Diffraction-Based Optimization;273
19.5;V. Materials and Methods;283
19.6;Acknowledgments;284
19.7;References;284
20;Chapter 11: Bacterial Membrane Proteins: The New Soluble Proteins?;290
20.1;I. Overview;290
20.2;II. Structures of Omps;291
20.3;III. Recombinant Production of Omps;305
20.4;IV. Recombinant Bacterial Inner Membrane Proteins and Eukaryotic Membrane Proteins;307
20.5;V. Crystallization of Membrane Proteins;308
20.6;VI. New Strategies for Eukaryotic Proteins from the Study of GPCR;309
20.7;VII. Summary;312
20.8;References;313
21;Index;320
22;Color Plate Section;327