Drosophila Cells in Culture

2. Auflage 2017
ISBN: 978-0-12-809473-0
Verlag: Elsevier Science

Buch, Englisch, 258 Seiten, Format (B × H): 151 mm x 233 mm, Gewicht: 220 g

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.

Zielgruppe

<p>early researchers, graduate students, investigators studying developmental biology, genetics, neuroscience, biochemistry and across the biological and biomedical sciences.</p>

Autoren/Hrsg.

Echalier, Guy

Perrimon, Norbert

Mohr, Stephanie E

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells2. Primary Cell Cultures of Drosophila Embryonic Cells3. Continuous Drosophila Cell Lines Established In Vitro4. Cells or Tissues In Course of Differentiation5. Hormonal Control of The Development of Insects: Implications In Organ or Cell Cultures of Drosophila6. Gene Transfer into Cultured and Applications7. Rnai and Crispr (Cas9, Cas9-Act, Cas9-Repressor) Methods to Perturb Gene Function8. Functional Genomics Screens Using Rnai, Crispr, and Orf Overexpression

Über Autor(innen)

Perrimon, Norbert
Norbert Perrimon is a leading researcher in developmental biology. His current research pertains to using Drosophilia as a model system to characterize the responses of cells to external signalling.

Echalier, Guy
Guy Echalier is an esteemed expert in the research of Drosophilia, having over thirty years experience handling in vitro cultured Drosophilia cells.

Mohr, Stephanie E
Stephanie E. Mohr is a leader in the field of high-throughput functional genomics screening in Drosophila cultured cells.