Activity-Based Proteomics

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2017
ISBN: 978-1-4939-8200-4
Verlag: Springer

Buch, Englisch, Band 1491, 222 Seiten, Paperback, Format (B × H): 178 mm x 254 mm, Gewicht: 506 g

Reihe: Methods in Molecular Biology

This volume focuses on explorative activity-based proteomics,biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Florea, Bogdan I.

Overkleeft, Herman S.

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Activity-Based Protein Profiling – From Chemical Novelty to Biomedical Stalwart.- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.- Inhibitor Discovery by Convolution Activity-Based Protein Profiling.- Activity-Based Protein Profiling in Bacteria.- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait.- Comparative Activity-Based Flavin-Dependent Oxidase Profiling.- Activity-Dependent Photoaffinity Labelling of Metalloproteases.- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes.- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools.- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe.- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha.- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells.- Cleavable Linkers in Chemical Proteomics Applications.- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.