Grandi Genomics, Proteomics and Vaccines

1. Auflage 2004
ISBN: 978-0-470-09392-4
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 336 Seiten, E-Book

While the sequence of the human genome sequence has hit theheadlines, extensive exploitation of this for practicalapplications is still to come. Genomic and post-genomictechnologies applied to viral and bacterial pathogens, which arealmost equally important from a scientific perspective, have thepotential to be translated into useful products and processes muchmore rapidly.
Genomics, Proteomics and Vaccines introduces the history ofvaccinology and discusses how vaccines are expected to evolve inthe future. It describes the relevant technologies, includinggenome sequencing and analysis, DNA microarrays, 2D electrophoresisand 2D chromatography, mass spectrometry and high-throughputprotein expression and purification. The book also featuresexamples of the exploitation of genomics and post-genomics invaccine discovery, and contains useful descriptions of the biologyand pathogenesis of clinically important bacterial pathogens.
This book should be of interest to all those working in vaccinediscovery and development in pharmaceutical and biotechnologycompanies as well as in academic institutions

Autoren/Hrsg.

Grandi, Guido

Inhaltsverzeichnis

Preface.
List of Contributors.
PART 1: INTRODUCTION.
1. Vaccination: Past, Present and Future (Maria Lattanzi andRino Rappuoli).
2. Bioinformatics, DNA Microarrays and Proteomics in VaccineDiscovery: Competing or Complementary Technologies? (GuidoGrandi).
PART 2: TECHNOLOGIES.
3. Genome Sequencing and Analysis (Hervé Tettelin andTamara Feldblyum).
4. Understanding DNA Microarrays: Sources and Magnitudes ofVariances in DNA Microarray Data Sets (She-pin Hung, G. WesleyHatfield, Suman Sundaresh and Pierre F. Baldi).
5. The proteome, Anno Domini Two Zero Zero Three (PierGiorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joël S.Rossier).
6. Mass Spectrometry in Proteomics (Pierre-Alain Binz).
7. High Throughput Cloning, Expression and PurificationTechnologies (Andreas Kreusch and Scott A. Lesley).
PART 3: APPLICATIONS.
8. Meningococcus B: from Genome to Vaccine (Davide Serruto, RinoRappuoli and Mariagrazia Pizza).
9. Vaccines Against Pathogenic Streptococci (John L. Telford,Immaculada Margarit y Ros, Domenico Maione, Vega Masignani,Hervé Tettelin, Giuliano Bensi and Guido Grandi).
10. Identification of the 'Antigenome'-a Novel Tools forDesign and Development of Subunit Vaccines Against BacterialPathogens (Eszter Nagy, Tamás Henics, Alexander von Gabain andAndreas Meinke).
11. Searching the Chlamydia Genomes for New Vaccine Candidates(Giulio Ratti, Oretta Finco and Guido Grandi).
12. Proteomics and Anti-Chlamydia Vaccine Discovery (GunnaChristiansen, Svend Birkelund, Brian B. Vandahl and Allan CShaw).
13. Proteome Analysis of Other Membrane and ExtracelularProteins from Pseudomonas aeruginosa for vaccine Discovery(Stuart J. Cordwell and Amanda S. Nouwens).
Index.