Gu / Wang Gene Discovery for Disease Models

1. Auflage 2011
ISBN: 978-0-470-93393-0
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 552 Seiten, E-Book

This book provides readers with new paradigms on the mutationdiscovery in the post-genome era. The completion of human and othergenome sequencing, along with other new technologies, such asmutation analysis and microarray, has dramatically accelerated theprogress in positional cloning of genes from mutated models. In2002, the Mouse Genome Sequencing Consortium stated that "Theavailability of an annotated mouse genome sequence now provides themost efficient tool yet in the gene hunter's toolkit. One can movedirectly from genetic mapping to identification of candidate genes,and the experimental process is reduced to PCR amplification andsequencing of exons and other conserved elements in the candidateinterval. With this streamlined protocol, it is anticipated thatmany decades-old mouse mutants will be understood precisely at theDNA level in the near future." The implication of such astatement should be similar to the identification of mutated genesfrom human diseases and animal models, when genome sequencing iscompleted for them. More than five years have passed, but genes inmany human diseases and animal models have not yet been identified.In some cases, the identification of the mutated genes has been abottleneck, because the genetic mechanism holds the key tounderstand the basis of the diseases. However, an integrativestrategy, which is a combination of genetic mapping, genomeresources, bioinformatics tools, and high throughput technologies,has been developed and tested. The classic paradigm of positionalcloning has evolved with completely new concepts of genomic cloningand protocols. This book describes new concepts of gene discoveryin the post-genome era and the use of streamlined protocols toidentify genes of interest. This book helps identify not only largeinsertions/deletions but also single nucleotide mutations orpolymorphisms that regulate quantitative trait loci (QTL).

Autoren/Hrsg.

Gu, W.

Wang, Yongjun

Inhaltsverzeichnis

Preface.
Acknowledgments.
Contributors.
1. Gene Discovery: From Positional Cloning to Genomic Cloning(Weikuan Gu and Daniel Goldowitz).
2. High-Throughput Gene Expression Analysis and theIdentification of Expression QTLs (Rudi Alberts and KlausSchughart).
3. DNA Methylation in the Pathogenesis of Autoimmunity(Xueqing Xu, Ping Yang, Zhang Shu, Yun Bai, and Cong-YiWang).
4. Cell-Based Analysis with Microfl uidic Chip (WangQi and Zhao Long).
5. Missing Dimension: Protein Turnover Rate Measurement inGene Discovery (Gary Guishan Xiao).
6. Bioinformatics Tools for Gene Function Prediction(Yan Cui).
7. Determination of Genomic Locations of Target Genetic Loci(Bo Chang).
8. Mutation Discovery Using High-Throughput MutationScreening Technology (Kai Li, Hanlin Gao, Hong-Guang Xie,Wanping Sun, and Jia Zhang).
9. Candidate Screening through Gene Expression Profile(Michal Korostynski).
10. Candidate Screening through High-Density SNP Array(Ching-Wan Lam and Kin-Chong Lau).
11. Gene Discovery by Direct Genome Sequencing (KunalRay, Arijit Mukhopadhyay, and Mainak Sengupta).
12. Candidate Screening through Bioinformatics Tools(Song Wu and Wei Zhao).
13. Using an Integrative Strategy to Identify Mutations(Yan Jiao and Weikuan Gu).
14. Determination of the Function of a Mutation(Bouchra Edderkaoui).
15. Confi rmation of a Mutation by Multiple MolecularApproaches (Hector Martinez-Valdez and BlancaOrtiz-Quintero).
16. Confi rmation of a Mutation by MicroRNA (HongweiZheng and Yongjun Wang).
17. Confi rmation of Gene Function Using TranslationalApproaches (Caroline J. Zeiss).
18. Confi rmation of Single Nucleotide Mutations(Jochen Graw).
19. Initial Identifi cation and Confi rmation of a QTL Gene(David C. Airey and Chun Li).
20. Gene Discovery of Crop Disease in the Postgenome Era(Yulin Jia).
21. Impact of Genomewide Structural Variation on GeneDiscovery (Lisenka E.L.M. Vissers and Joris A.Veltman).
22. Impact of Whole Genome Protein Analysis on Gene Discoveryof Disease Models (Sheng Zhang, Yong Yang, and Theodore W.Thannhauser).
Index.

Über Autor(innen)

Weikuan Gu received his PhD from Cornell University in 1994and joined the University of Tennessee Health Science Center as anassistant professor in 2002. Dr. Gu's lab has developed anintegrated strategy for the positional cloning of genes, a strategywhich has been successfully applied to clone several genes fromspontaneous mouse mutations.
Yongjun Wang received his MD from Hebei Medical Collegein 1982 and his MBA from Peking University in 2004. Dr. Wang joinedBeijing Tiantan Hospital in 2000 and now serves as the vicepresident of the hospital.