Membrane Protein Purification and Crystallization

A Practical Guide
2. Auflage 2002
ISBN: 978-0-12-361776-7
Verlag: Elsevier Science

Buch, Englisch, 316 Seiten, Format (B × H): 205 mm x 239 mm, Gewicht: 599 g

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Zielgruppe

Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry

Autoren/Hrsg.

Hunte, Carola

Jagow, Gebhard von

Schagger, Hermann

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Physik Angewandte Physik Biophysik
• Naturwissenschaften Biowissenschaften Angewandte Biologie Biophysik
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

I. Strategies and Techniques Purification Strategies for Membrane Proteins Chromatographic Techniques and Basic Operations in Membrane Protein Purification Production and purification of recombinant membrane proteins SDS Electrophoresis Techniques Blue-Native Electrophoresis Preparative Isoelectric Focussing Membrane protein crystallization

II. Discussion of Selected Isolation Protocols Lipid Dependent Inactivation and Reactivation of Bovine complex III Purification of affinity-epitope tagged G-protein coupled receptors Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D Crystallization Purification of the yeast cytochrome bc1 complex

III. Crystallization of Membrane Proteins Antibody fragment-mediated crystallization of membrane proteins Crystallization of Wolinella succinogenes quinol:fumarate reductase Ba3-type cytochrome-c oxidase from Thermus thermophilus purification, crystallization, and crystal transformation Two-dimensional crystallization of membrane proteins: a practical guide In cubo crystallization of membrane proteins

Über Autor(innen)

Schagger, Hermann
Deceased: February 12, 2017